Uricase Antibody

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Western Blot: Uricase Antibody [NB600-934] - Analysis using HRP conjugate of NB600-934. Lane 1: purified Uricase under reducing conditions. Lane 2: purified Uricase under reducing conditions. Lane 3: purified Uricase ...read more
Western Blot: Uricase Antibody [NB600-934] - Analysis using the HRP conjugate of NB600-934. Detection of Lane 1: purified Uricase under reducing conditions. Detection of Lane 2: purified Uricase under reducing ...read more
Western Blot: Uricase Antibody [NB600-934] - Detection of purified Uricase under reducing and non-reducing conditions. Samples of 1 and 0.25 ug of protein per lane were run by SDS-PAGE and reduced samples of purified ...read more

Product Details

Summary
Reactivity BaSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price

Uricase Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Full length native protein: Uricase (Bacillus Genus)
Localization
Cytoplasmic
Specificity
Recognizes uricase.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
UOX
Purity
Ion exchange chromatography
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100
Application Notes
This product has been assayed against 1.0 ug of uricase [Bacillus species] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:7,000 - 1:30,000 of the reconstitution concentration is suggested for this product.
Publications
Read Publication using NB600-934.

Reactivity Notes

Cross-reacts with Bacillus Genus. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Ion exchange chromatography

Alternate Names for Uricase Antibody

  • UOXP
  • urate oxidase, pseudogene
  • URICASE

Background

The enzyme urease catalyzes the hydrolysis of urea to create carbon dioxide and ammonia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Uricase Antibody (NB600-934)(1)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol UOX