Uricase Antibody

Western Blot: Uricase Antibody [NB600-934] - Detection of purified Uricase under reducing and non-reducing conditions. Samples of 1 and 0.25 ug of protein per lane were run by SDS-PAGE and reduced samples of purified Uricase contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Goat anti Uricase. Primary antibody was detected with Dylight 649 conjugated Donkey anti Goat 1:10K 1.5 hr RT in and imaged on the VersaDoc imaging system.

Western Blot: Uricase Antibody [NB600-934] - Analysis using the HRP conjugate of NB600-934. Detection of Lane 1: purified Uricase under reducing conditions. Detection of Lane 2: purified Uricase under reducing conditions. Detection of Lane 3: purified Uricase under non-reducing conditions. Detection of Lane 4: purified Uricase under non-reducing conditions Load: ~1.0 ug (Detection of Lane 1 and 3) and 0.25 ug (Detection of Lane 2 and 4). Primary antibody: Uricase Peroxidase conjugated Antibody at 1:5,000 overnight at 4C. Secondary antibody: DyLight (TM) 649 goat secondary antibody at 1:10,000 for 90 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 58 kDa, 58 kDa for Uricase. Other band(s): Uricase splice variants and isoforms.

• Reactivity: Ba
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Uricase Antibody Summary

• Immunogen: Uricase [Bacillus species] (Uniprot: Q5WBJ3)
• Localization: Cytoplasmic
• Specificity: Cross reactivity against Uricase from other sources is unknown.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UOX
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:2500
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:1000
• Application Notes: This product has been tested by western blot and is assayed against 1.0 ug of Uricase [Bacillus species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:3,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Reactive with Uricase from Bacillus species.
Cross reactivity against Uricase from other sources is unknown

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Notes

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Uricase Antibody

• UOXP
• urate oxidase, pseudogene
• URICASE

Background

The enzyme urease catalyzes the hydrolysis of urea to create carbon dioxide and ammonia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Uricase Antibody (NB600-934)(1)

Publication using NB600-934

• Chang YJ, Chang CH. Intelligent Computation for Optimal Fabrication Condition of a Protein Chip with Ni-Co Alloy-Coated Surface. J Lab Autom. 2015-03-23 [PMID: 25800959]

Bioinformatics

• Gene Symbol: UOX
• Uniprot:
  • Q5WBJ3(Human)