Glut1 Antibody (202921) [Unconjugated]

Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glut1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Glut1 at approximately 69 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 202921
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Glut1 Antibody (202921) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Glut1
  Met1-Val492
  Accession # AAA52571
• Specificity: Detects human Glut1 in ELISAs.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC2A1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glut1 Antibody (202921) [Unconjugated]

• Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis)
• CSE
• DYT17
• DYT18
• DYT9
• EIG12
• Glucose transporter type 1, erythrocyte/brain
• Glut1
• GLUT-1
• GLUT1DS
• HepG2 glucose transporter
• HTLVR
• Human T-Cell Leukemia Virus (I and II) Receptor
• MGC141895
• MGC141896
• PED
• SLC2A1
• solute carrier family 2 (facilitated glucose transporter), member 1
• Solute Carrier Family 2 Member 1
• solute carrier family 2, facilitated glucose transporter member 1

Background

Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8⁺ T cells, and erythrocytes (1‑5). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood‑brain barrier.

1. Mueckler, M. et al. 1994, Eur. J. Biochem. 219:713.
2. Meuckler, M. et al. 1985, Science 229:941.
3. Jones, K.S. et al. 2006, J. Virol. 8291.
4. Takenouchi, N. et al. 2007, J. Virol. 1506.
5. Kinet, S. et al. 2007, Retrovirology 4:31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glut1 Antibody (MAB14181)(3)

Publications using MAB14181

• Liangyu Cai, Xin Jin, Jiannan Zhang, Le Li, Jinfeng Zhao Metformin suppresses Nrf2-mediated chemoresistance in hepatocellular carcinoma cells by increasing glycolysis Aging (Albany NY) 2020-09-14 [PMID: 32927432]
• Liangyu Cai, Xin Jin, Jiannan Zhang, Le Li, Jinfeng Zhao Metformin suppresses Nrf2-mediated chemoresistance in hepatocellular carcinoma cells by increasing glycolysis Aging (Albany NY) 2020-09-14 [PMID: 32927432] (Western Blot, Human)
• Nemanja Milićević, Jacoline B. ten Brink, Anneloor L.M.A. ten Asbroek, Arthur A. Bergen, Marie-Paule Felder-Schmittbuhl The circadian clock regulates RPE-mediated lactate transport via SLC16A1 (MCT1) Experimental Eye Research 2020-01-01 [PMID: 31678436]

Bioinformatics

• Gene Symbol: SLC2A1
• Uniprot:
  • AAA52571()