Glut1 Antibody (202915) [Unconjugated]

Images

 
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed ...read more
Jurkat human acute T cell leukemia cell line either (A) untreated or (B) cultured in nutrient-depleted media was stained with Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418, filled histogram) or isotype ...read more
Glut1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
202915
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Glut1 Antibody (202915) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human Glut1
Met1-Val492
Accession # AAA52571
Specificity
Detects human Glut1. Stains human Glut1-transfected NS0 cells, but not NS0 control transfectants. Although Human Glut1 Antibody detects Glut1 on the surface of T cells (1, 2), it does not detect it on erythrocytes (5). The reason for this discrepancy is not understood, but may be related to conformational or post-translational modification differences.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SLC2A1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
MAB1418 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glut1 Antibody (202915) [Unconjugated]

  • Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis)
  • CSE
  • DYT17
  • DYT18
  • DYT9
  • EIG12
  • Glucose transporter type 1, erythrocyte/brain
  • Glut1
  • GLUT-1
  • GLUT1DS
  • HepG2 glucose transporter
  • HTLVR
  • Human T-Cell Leukemia Virus (I and II) Receptor
  • MGC141895
  • MGC141896
  • PED
  • SLC2A1
  • solute carrier family 2 (facilitated glucose transporter), member 1
  • Solute Carrier Family 2 Member 1
  • solute carrier family 2, facilitated glucose transporter member 1

Background

Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8+ T cells, and erythrocytes (1‑4). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood‑brain barrier.

  1. Mueckler, M. et al. 1994, Eur. J. Biochem. 219:713.
  2. Meuckler, M. et al. 1985, Science 229:941.
  3. Jones, K.S. et al. 2006, J. Virol. 8291.
  4. Takenouchi, N. et al. 2007, J. Virol. 1506.
  5. Kinet, S. et al. 2007, Retrovirology 4:31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glut1 Antibody (MAB1418)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Flow Cytometry, ICC, IHC.


Filter By Application
Flow Cytometry
(9)
ICC
(1)
IHC
(2)
All Applications
Filter By Species
Human
(11)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using MAB1418 Applications Species
R Alföldi, JÁ Balog, N Faragó, M Halmai, E Kotogány, P Neuperger, LI Nagy, LZ Fehér, GJ Szebeni, LG Puskás Single Cell Mass Cytometry of Non-Small Cell Lung Cancer Cells Reveals Complexity of In vivo And Three-Dimensional Models over the Petri-dish Cells, 2019;8(9):. 2019 [PMID: 31527554] (Flow Cytometry, Human) Flow Cytometry Human
GA Gualdoni, KA Mayer, AM Kapsch, K Kreuzberg, A Puck, P Kienzl, F Oberndorfe, K Frühwirth, S Winkler, D Blaas, GJ Zlabinger, J Stöckl Rhinovirus induces an anabolic reprogramming in host cell metabolism essential for viral replication Proc. Natl. Acad. Sci. U.S.A., 2018;115(30):E7158-E7165. 2018 [PMID: 29987044] (Flow Cytometry, Human) Flow Cytometry Human
D Vignali, E Cantarelli, C Bordignon, A Canu, A Citro, A Annoni, L Piemonti, P Monti Detection and Characterization of CD8+ Autoreactive Memory Stem T Cells in Patients with Type 1 Diabetes Diabetes, 2018;0(0):. 2018 [PMID: 29506985] (IHC, Human) IHC Human
SK Varanasi, D Donohoe, U Jaggi, BT Rouse Manipulating Glucose Metabolism during Different Stages of Viral Pathogenesis Can Have either Detrimental or Beneficial Effects J. Immunol., 2017;0(0):. 2017 [PMID: 28768727] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Emerging Role and Characterization of Immunometabolism: Relevance to HIV Pathogenesis, Serious Non-AIDS Events, and a Cure J Immunol, 2016;196(11):4437-44. 2016 [PMID: 27207806] (Flow Cytometry, Human) Flow Cytometry Human
Basu S, Hubbard B, Shevach E Foxp3-mediated inhibition of Akt inhibits Glut1 (glucose transporter 1) expression in human T regulatory cells. J Leukoc Biol, 2015;97(2):279-83. 2015 [PMID: 25492937] (Flow Cytometry, Human) Flow Cytometry Human
Palmer C, Ostrowski M, Gouillou M, Tsai L, Yu D, Zhou J, Henstridge D, Maisa A, Hearps A, Lewin S, Landay A, Jaworowski A, McCune J, Crowe S Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection. AIDS, 2014;28(3):297-309. 2014 [PMID: 24335483] (Flow Cytometry, Human) Flow Cytometry Human
Chan O, Burke J, Gao D, Fish E The chemokine CCL5 regulates glucose uptake and AMP kinase signaling in activated T cells to facilitate chemotaxis. J Biol Chem, 2012;287(35):29406-16. 2012 [PMID: 22782897] (Flow Cytometry, Human) Flow Cytometry Human
Tanaka A, Jinno-Oue A, Shimizu N Entry of human T-cell leukemia virus type 1 is augmented by heparin sulfate proteoglycans bearing short heparin-like structures. J. Virol., 2012;86(6):2959-69. 2012 [PMID: 22238310] (Flow Cytometry, Human) Flow Cytometry Human
Han M, Lee H, Cho K, Kim J, Roh J, Choi S, Nam S, Kim S Role of FDG-PET as a biological marker for predicting the hypoxic status of tongue cancer. Head Neck, 0;34(10):1395-402. 0 [PMID: 22052623] (IHC, Human) IHC Human
Show All 12 Publications.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB1418U
Alexa Fluor 405 FAB1418V
Alexa Fluor 488 FAB1418G
Alexa Fluor 594 FAB1418T
Alexa Fluor 647 FAB1418R
Alexa Fluor 700 FAB1418N
Alexa Fluor 750 FAB1418S
Allophycocyanin FAB1418A
Fluorescein FAB1418F
Phycoerythrin FAB1418P

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Blogs on Glut1.

Deficiency of GluT1 leads to neurological problems while excess is involved in cancers
By Jamshed Arslan, Pharm. D., PhD. What Are GluTs?Mammalian cell metabolism is incomplete without glucose   . Glucose is a monosaccharide that is transported to the cells through facilitative diffusion, a ...  Read full blog post.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

Forecasting and Targeting a Rare Cancer with Hypoxia-Inducible Factor
By Jamshed Arslan Pharm.D. Cancers of nerve, adipose, and other soft tissues are called soft tissue sarcomas (STS). Malignant peripheral nerve sheath tumor (MPNST) is an example of a rare and hard-to-treat STS; eve...  Read full blog post.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

Glucose Transporter 1 (GLUT1): a Key Metabolic Neuronal Player
Glucose is the principal fuel source for the brain and GLUT1 is the only vehicle by which glucose enters the brain. In case of GLUT1 deficiency, the risk of clinical manifestations is increased in infancy and childhood, when the brain glucose demand i...  Read full blog post.

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Bioinformatics

Gene Symbol SLC2A1
Uniprot