GluR2 Antibody

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

GluR2 Antibody Summary

• Immunogen: A synthetic peptide from extracellular domain of human GluR2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GRIA2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:300-1:2000
  • Immunohistochemistry-Frozen 1:300-1:2000
  • Immunoprecipitation
  • Western Blot 1:300-1:2000
• Application Notes: The optimal dilution should be determined by the end user. Use in immunoprecipitation reported in scientific literature (PMID: 27461448). Use in ICC/IF, and IHC-FR reported in scientific literature (PMID: 30150703).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for GluR2 Antibody

• AMPA 2
• AMPA2
• GluA2
• GluR2
• GLURB
• GluR-K2
• glutamate receptor, ionotropic, AMPA 2
• GRIA2
• HBGR2

Background

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The ionotropic glutamate receptors are multimeric assemblies of four or five subunits, and are subdivided into three groups (AMPA, NMDA and Kainate receptors) based on their pharmacology structural similarities. The ionotropic receptors are ligand gated ion channels, i.e. on binding glutamate that has been released from a companion cell, charged ions such as Na+ and Ca2+ pass through a channel in the centre of the receptor complex. This flow of ions results in a depolarisation of the plasma membrane and the generation of an electrical current that is propagated down the processes (dendrites and axons) of the neuron to the next in line.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GluR2 Antibody (NBP1-46490)(3)

Publications using NBP1-46490

• Lee FHF, Zhang H, Jiang A et al. Specific Alterations in Astrocyte Properties via the GluA2-GAPDH Complex Associated with Multiple Sclerosis Sci Rep 2018-08-27 [PMID: 30150703] (ICC/IF, IHC-Fr, Mouse)
• Frankie Hang Fung Lee, Ping Su, Yu-Feng Xie et al. Disrupting GluA2-GAPDH Interaction Affects Axon and Dendrite Development. Scientific Reports 2016-07-27 [PMID: 27461448] (IP, Mouse)
• Blocking GluR2-GAPDH ameliorates experimental autoimmune encephalomyelitis Zhai D, Lee FHF, D'Souza C Ann Clin Transl Neurol [PMID: 25909084] (WB, IP, Mouse)

Bioinformatics

• Gene Symbol: GRIA2
• Entrez:
  • 2891(Human)