GluR2 [p Ser880] Antibody - Azide Free

Western Blot: GluR2 [p Ser880] Antibody [NBP1-28662] - Rat brain homogenate showing specific immunolabeling of the ~100k GluR2 protein phosphorylated at Ser880 (control). immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).

• Reactivity: Rt, Hu, Mu, Ch, Pm, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

GluR2 [p Ser880] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit GluR2 [p Ser880] Antibody - Azide Free (NBP1-28662) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser880 conjugated to KLH. Accession # P19491
• Modification: p Ser880
• Specificity: Specific for endogenous levels of the ~100 kDa GluR2 protein phosphorylated at Ser880. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
• Predicted Species: Human (100%), Mouse (100%), Zebrafish (100%), Chicken (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRIA2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for GluR2 [p Ser880] Antibody - Azide Free

• AMPA 2
• AMPA2
• GluA2
• GluR2
• GLURB
• GluR-K2
• glutamate receptor, ionotropic, AMPA 2
• GRIA2
• HBGR2

Background

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinanen et al., 1990; Hollmann and Heinemann, 1994). The number of GluR2 subunits in the AMPA receptor complex affects the Ca2+ permeability, rectification and single-channel conductance of AMPA receptors. Ser880 has been identified as the PKC phosphorylation site within the C-terminal region of GluR2 and has been shown to differentially regulate the interaction of the PDZ domain-containing proteins GRIP1 and PICK 1 (Matsuda et al., 1999)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRIA2
• Uniprot:
  • P19491(Rat)