GluR1 Antibody

Product Discontinued

GluR1 Antibody Summary

• Immunogen: Synthetic peptide - human, C terminal. The immunogen sequence is located within amino acids 816-906 of P42261 (UniProt).
• Localization: Integral membrane protein
• Specificity: metabotropic Glutamate Receptor 1a
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRIA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: IHC-P: Use at a dilution of 1/50 for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. NB120-15303 demonstrates cytoplasmic staining. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Predicted molecular weight: 143 kDa
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4) and BSA
• Preservative: 0.1% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GluR1 Antibody

• AMPA 1
• AMPA-selective glutamate receptor 1
• GluA1
• GLUH1
• GluR1
• GluR-1
• GLUR1gluR-A
• GLURA
• GluR-A
• gluR-K1
• glutamate receptor 1
• Glutamate receptor ionotropic, AMPA 1
• glutamate receptor, ionotropic, AMPA 1
• GRIA1
• HBGR1
• MGC133252

Background

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The GRIA1 belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Each of the members (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing. The receptor subunits encoded by each isoform vary in their signal transduction properties. The isoform presented here is the flop isoform. In situ hybridization experiments showed that human GRIA1 mRNA is present in granule and pyramidal cells in the hippocampal formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRIA1
• Entrez:
  • 2890(Human)
  • 14799(Mouse)
  • 471714(Rat)
• Uniprot:
  • P23818(Mouse)
  • P19490(Rat)