GluR1 Antibody (RH95) - Azide Free

Western Blot: GluR1 Antibody (RH95) [NBP2-29534] - Rat hippocampal lysate showing specific immunolabeling of the ~105k GluR1 protein.

• Reactivity: Mu, Rt
• Applications: WB
• Clone: RH95
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide Free

GluR1 Antibody (RH95) - Azide Free Summary

• Description: Novus Biologicals Mouse GluR1 Antibody (RH95) - Azide Free (NBP2-29534) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # P19490
• Specificity: Specific for endogenous levels of the ~105 kDa GluR1 protein.
• Preparation Method: Protein G purified culture supernatant
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRIA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

The antibody has been directly tested for reactivity in Western blots with mouse and rat tissue. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for GluR1 Antibody (RH95) - Azide Free

• AMPA 1
• AMPA-selective glutamate receptor 1
• GluA1
• GLUH1
• GluR1
• gluR-1
• GLUR1gluR-A
• GLURA
• GluR-A
• gluR-K1
• glutamate receptor 1
• Glutamate receptor ionotropic, AMPA 1
• glutamate receptor, ionotropic, AMPA 1
• GRIA1
• HBGR1
• MGC133252

Background

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinanen et al., 1990;Hollmann and Heinemann, 1994).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRIA1
• Entrez:
  • 50592(Rat)
• Uniprot:
  • P19490(Rat)