Glucose 6 Phosphate Dehydrogenase Antibody (2F6)

Images

 
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2F6) [NBP1-30358] - Lane 1. MCF cell lysate, Lane 2. HeLa cell lysate, Lane 3. Jurkat cell lysate.
Immunocytochemistry/ Immunofluorescence: Glucose 6 Phosphate Dehydrogenase Antibody (2F6) [NBP1-30358] - Analysis in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human G6PD antibody ...read more
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (AT2F6) [NBP1-30358] - Cell lysates of MCF7 (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human G6PD (1:1000). Proteins were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
2F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Glucose 6 Phosphate Dehydrogenase Antibody (2F6) Summary

Immunogen
Recombinant human G6PD (35-506aa) purified from E. coli
Marker
Cytosol Marker
Specificity
This antibody is specific for human G6PD. This monoclonal antibody, clone 2F6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human G6PD protein.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
G6PD
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000-1:2000.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Glucose 6 Phosphate Dehydrogenase Antibody (2F6)

  • G6PD
  • G6PD1EC 1.1.1.49
  • glucose-6-phosphate dehydrogenase

Background

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). This full-length 545 aa form has been reported to be inactive, but may be processed to the smaller (515 aa) active form. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glucose 6 Phosphate Dehydrogenase Antibody (NBP1-30358) (0)

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Bioinformatics

Gene Symbol G6PD
Entrez
Uniprot