Glucokinase/GCK Antibody (849520)

Images

 
Western blot shows lysates of Huh‑7 human hepatoma cell line, Hepa 1‑6 mouse hepatoma cell line, and HT‑2 mouse T cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Glucokinase/GCK Monoclonal ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
849520
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Glucokinase/GCK Antibody (849520) Summary

Immunogen
E. coli-derived recombinant human Glucokinase/GCK
Val16-Gln465
Accession # P35557
Specificity
Detects human Glucokinase/GCK in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human PRF-1 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GCK
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glucokinase/GCK Antibody (849520)

  • EC 2.7.1
  • EC 2.7.1.2
  • FGQTL3
  • GCK
  • GK
  • GLK
  • glucokinase (hexokinase 4)
  • Glucokinase
  • Hexokinase-4
  • Hexokinase-D
  • HHF3
  • HK4
  • HK4pancreatic isozyme
  • HKIV
  • HXKP
  • LGLK
  • maturity onset diabetes of the young 2
  • MODY2

Background

Hexokinases phosphorylate hexose to form hexose 6‑phosphate, the first step for hexose metabolism. There are four mammalian hexokinases (I, II, III and IV) and hexokinase IV is commonly known as glucose kinase (GCK). Unlike hexokinase I, II and III, which have high affinity for glucose and are strongly inhibited by the product, glucose‑6‑phosphate (1), GCK has much lower affinity for glucose and is not inhibited by the product (2, 3). Consequently, GCK has a Km for glucose of approximately 7 mM (4), which is 100 times higher than that of hexokinase I, II, and III. This unique enzymatic property of GCK allows it to respond to blood glucose levels and contribute to the maintenance of blood glucose levels within the normal physiological range of 4 mM to 6 mM. In the pancreatic islets, GCK serves as a glucose sensor to control insulin release in the beta cells, and to control glucagon release in the alpha cells (5). In hepatocytes, GCK responds to changes of ambient glucose levels by increasing or reducing glycogen synthesis. Mutations in GCK have been associated with non‑insulin‑dependent diabetes mellitus (6, 7), maturity‑onset diabetes of the young type 2 (8), and hyperinsulinemia of infancy (9). The enzyme activity was measured using a phosphatase coupled kinase assay (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GCK
Entrez
Uniprot