GIPR Antibody (591853) [Unconjugated]

F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins." > F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins." title="HEK293 human embryonic kidney cell line transfected with either (A) human GIPR or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins." />

HEK293 human embryonic kidney cell line transfected with either (A) human GIPR or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="GIPR was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

GIPR was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

2257, 25 ng/mL) binds to Human GIPR-transfected HEK293 cells (black dotted line). Binding is completely blocked (orange histogram) by 2.5 µg/mL of Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210). Mouse IgG₁Isotype Control (Catalog # MAB002) at 2.5 µg/mL was used as a control (blue line). Cells were stained with Sterptavidin-APC (Catalog # F0050)." > 2257, 25 ng/mL) binds to Human GIPR-transfected HEK293 cells (black dotted line). Binding is completely blocked (orange histogram) by 2.5 µg/mL of Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210). Mouse IgG₁Isotype Control (Catalog # MAB002) at 2.5 µg/mL was used as a control (blue line). Cells were stained with Sterptavidin-APC (Catalog # F0050)." title="In a functional flow cytometry test, biotinylated recombinant Human GIP (Catalog # 2257, 25 ng/mL) binds to Human GIPR-transfected HEK293 cells (black dotted line). Binding is completely blocked (orange histogram) by 2.5 µg/mL of Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210). Mouse IgG₁Isotype Control (Catalog # MAB002) at 2.5 µg/mL was used as a control (blue line). Cells were stained with Sterptavidin-APC (Catalog # F0050)." />

In a functional flow cytometry test, biotinylated recombinant Human GIP (Catalog # <a class=

• Reactivity: Hu
• Applications: Flow, IHC, B/N, CyTOF-ready
• Clone: 591853
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

GIPR Antibody (591853) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human GIPR.
  Met1-Cys466
  Accession # P48546
• Specificity: Detects human GIPR in direct ELISAs. Stains human GIPR transfected cells but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GIPR
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction 2.5 ug/mL
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GIPR Antibody (591853) [Unconjugated]

• gastric inhibitory polypeptide receptor
• GIPR
• GIP-R
• Glucose-dependent insulinotropic polypeptide receptor
• MGC126722
• PGQTL2

Background

GIPR is a 7-transmembrane receptor for GIP (glucose-dependent insulinotropic polypeptide or gastric inhibitory polypeptide). The 466 amino acid (aa) human GIPR contains 176 extracellular domain (ECD) aa that share 77% and 81% aa identity with mouse and rat GIPR ECD, respectively. A splice isoform of 430 aa has a deletion of aa 58-93 in the N-terminal ECD, while isoforms of 491 and 419 aa have alternate C-terminal cytoplasmic sequences. Engagement by GIP on pancreatic b‑cells activates adenylate cyclase to regulate insulin compensation in the presence of high circulating glucose. GIPR is also expressed on adipocytes, osteoblasts and myelinating Schwann cell membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GIPR Antibody (MAB8210)(2)

Publications using MAB8210

• Shu-Chen Lu, Michelle Chen, Larissa Atangan, Elizabeth A. Killion, Renee Komorowski, Yuan Cheng et al. GIPR antagonist antibodies conjugated to GLP-1 peptide are bispecific molecules that decrease weight in obese mice and monkeys Cell Reports Medicine 5/1/2021 [PMID: 34095876]
• EA Killion, M Chen, JR Falsey, G Sivits, T Hager, L Atangan, J Helmering, J Lee, H Li, B Wu, Y Cheng, MM Véniant, DJ Lloyd Chronic glucose-dependent insulinotropic polypeptide receptor (GIPR) agonism desensitizes adipocyte GIPR activity mimicking functional GIPR antagonism Nat Commun, 2020-10-05;11(1):4981. 2020-10-05 [PMID: 33020469] (ICC, Mouse)

Bioinformatics

• Gene Symbol: GIPR
• Uniprot:
  • P48546()