GLI-1 Antibody [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB, IHC, ChIP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 700

GLI-1 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human GLI-1
  Met1-Glu234
  Accession # P08151
• Specificity: Detects human GLI-1 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human GLI-3 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GLI-1 Antibody [Alexa Fluor® 700]

• GLI family zinc finger 1
• GLI1
• GLI-1
• glioma-associated oncogene family zinc finger 1
• glioma-associated oncogene homolog 1 (zinc finger protein)
• Glioma-associated oncogene
• GLIzinc finger protein GLI1
• Oncogene GLI

Background

GLI-1 is a transcription regulator with 5 conserved tandem C2-H2 zinc finger domains, joined by conserved histidine/cysteine linkers. It is a known oncogene that was originally isolated in a human glioblastoma. GLI-1 acts downstream of Hedgehog signaling and is involved in cell proliferation and pattern formation during embryonic development. Within the region used as the immunogen, human GLI-1 shares 81% amino acid sequence homology with mouse GLI-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.