GLI-1 Antibody (OTI4E2) - Azide and BSA Free

Images

 
Western Blot: GLI-1 Antibody (OTI4E2) - Azide and BSA Free [NBP2-70831] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1.
Immunohistochemistry: GLI-1 Antibody (OTI4E2) - Azide and BSA Free [NBP2-70831] - Analysis of Human skin tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: GLI-1 Antibody (OTI4E2) - Azide and BSA Free [NBP2-70831] - Analysis of Human adult brain tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: GLI-1 Antibody (OTI4E2) - Azide and BSA Free [NBP2-70831] - Analysis of Human adult heart tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: GLI-1 Antibody (OTI4E2) - Azide and BSA Free [NBP2-70831] - Analysis of Human embryonic brain cortex tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: GLI-1 Antibody (OTI4E2) - Azide and BSA Free [NBP2-70831] - Analysis of Human embryonic cerebellum tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: GLI-1 Antibody (OTI4E2) - Azide and BSA Free [NBP2-70831] - Analysis of Human embryonic liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: GLI-1 Antibody (OTI4E2) - Azide and BSA Free [NBP2-70831] - Analysis of Human melanoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: GLI-1 Antibody (OTI4E2) - Azide and BSA Free [NBP2-70831] - Analysis of Human muscle tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: GLI-1 Antibody (OTI4E2) - Azide and BSA Free [NBP2-70831] - Analysis of Human placenta tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: GLI-1 Antibody (OTI4E2) - Azide and BSA Free [NBP2-70831] - Analysis of Human testicular cancer tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, KD
Clone
OTI4E2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

GLI-1 Antibody (OTI4E2) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 755-1106 of human GLI1 (NP_005260) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GLI1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunohistochemistry 1:150
  • Knockdown Validated
Theoretical MW
117.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for GLI-1 Antibody (OTI4E2) - Azide and BSA Free

  • GLI family zinc finger 1
  • GLI1
  • GLI-1
  • glioma-associated oncogene family zinc finger 1
  • glioma-associated oncogene homolog 1 (zinc finger protein)
  • Glioma-associated oncogene
  • GLIzinc finger protein GLI1
  • Oncogene GLI

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70831AF350
Alexa Fluor 405 NBP2-70831AF405
Alexa Fluor 488 NBP2-70831AF488
Alexa Fluor 532 NBP2-70831AF532
Alexa Fluor 594 NBP2-70831AF594
Alexa Fluor 647 NBP2-70831AF647
Alexa Fluor 700 NBP2-70831AF700
Alexa Fluor 750 NBP2-70831AF750
Biotin NBP2-70831B
DyLight 350 NBP2-70831UV
DyLight 405 NBP2-70831V
DyLight 488 NBP2-70831G
DyLight 550 NBP2-70831R
DyLight 594 NBP2-70831DL594
DyLight 650 NBP2-70831C
DyLight 680 NBP2-70831FR
DyLight 755 NBP2-70831IR
FITC NBP2-70831F
HRP NBP2-70831H
Janelia Fluor 549 NBP2-70831JF549
Janelia Fluor 646 NBP2-70831JF646

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Bioinformatics

Gene Symbol GLI1