Human GITR/TNFRSF18 ELISA Kit (Colorimetric)

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Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1719
    • Availability
      Product Discontinued

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Human GITR/TNFRSF18 ELISA Kit (Colorimetric) Summary

Sensitivity
15 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TNFRSF18

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
TNFRSF18 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNFRSF18.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A (Item D): Diluent buffer, 0.09% sodium azide as preservative.
  2. Assay Diluent B (Item E): 5x concentrated buffer.
  3. Detection Antibody GITR (Item F): Biotinylated anti-human GITR (each vial isenough to assay half the microplate).
  4. GITR Microplate (Item A): Coated with anti-human GITR.
  5. HRP-Streptavidin Concentrate (Item G): 400x concentrated HRP-conjugatedstreptavidin.
  6. Standard Protein (Item C): Human GITR. 1 vials is enough to run eachstandard in duplicate.
  7. Stop Solution (Item I): 0.2 M sulfuric acid.
  8. TMB One-Step Substrate Reagent (Item H): 3,3’,5,5’-tetramethylbenzidine(TMB) in buffered solution.
  9. Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human GITR/TNFRSF18 ELISA Kit (Colorimetric)

  • Activation-inducible TNFR family receptor
  • AITR
  • AITRTNF receptor superfamily activation-inducible protein
  • CD357 antigen
  • CD357
  • GITR
  • GITR-D
  • GITRtumor necrosis factor receptor superfamily member 18
  • Glucocorticoid-induced TNFR-related protein
  • TNFRSF18
  • tumor necrosis factor receptor superfamily, member 18

Background

GITR is encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for GITR/TNFRSF18 ELISA Kit (KA1719). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol TNFRSF18