Human GITR/TNFRSF18 ELISA Kit (Colorimetric) Summary
| Sensitivity |
15 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
TNFRSF18 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
TNFRSF18 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNFRSF18. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Assay Diluent A (Item D): Diluent buffer, 0.09% sodium azide as preservative.
- Assay Diluent B (Item E): 5x concentrated buffer.
- Detection Antibody GITR (Item F): Biotinylated anti-human GITR (each vial isenough to assay half the microplate).
- GITR Microplate (Item A): Coated with anti-human GITR.
- HRP-Streptavidin Concentrate (Item G): 400x concentrated HRP-conjugatedstreptavidin.
- Standard Protein (Item C): Human GITR. 1 vials is enough to run eachstandard in duplicate.
- Stop Solution (Item I): 0.2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 3,3’,5,5’-tetramethylbenzidine(TMB) in buffered solution.
- Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human GITR/TNFRSF18 ELISA Kit (Colorimetric)
Background
GITR is encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for GITR/TNFRSF18 ELISA Kit (KA1719). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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