GITR/TNFRSF18 Antibody [Biotin]

• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

GITR/TNFRSF18 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human GITR/TNFRSF18
  Gln26-Glu161 (Thr45Ala)
  Accession # Q9Y5U5
• Specificity: Detects human GITR/TNFRSF18 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant mouse GITR, recombinant human (rh) 4-1BB, rhFas, rhHVEM, rhDR6, rhOPG, rhGITR Ligand, and rhCD27 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNFRSF18
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Application Notes: ELISA Capture: Human GITR/TNFRSF18 Antibody (Catalog # MAB689)
  ELISA Detection: Human GITR/TNFRSF18 Biotinylated Antibody (Catalog # BAF689)
  Standard: Recombinant Human GITR/TNFRSF18 Fc Chimera (Catalog # 689-GR)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

GITR (glucocorticoid-induced tumor necrosis factor receptor, also named AITR, activation-inducible TNF R family member), is a 228 amino acid (aa) type I transmembrane protein belonging to the TNF R family and has been designated TNFRSF18. The GITR cytoplasmic domain has striking homology with the cytoplasmic domain of 4-1BB and CD27. Human GITR shares 55% homology with murine GITR. GITR is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is up-regulated by antigen stimulation or by treatment with anti-CD3 plus anti-CD28, or PMA plus ionomycin. Human GITR ligand was identified from human umbilical vein endothelial cells and is a 177 aa polypeptide belonging to the TNF superfamily (TNFSF18). Ligation of GITR can activate NF-kappa B through TRAF2, and protect T cells from TCR activation-induced cell death. It has been proposed that GITR ligand and GITR may modulate T lymphocyte functions.

1. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
2. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
3. Gurney, A.L. et al. (1999) Current Biology 9:215.
4. Kwon, B. et al. (1999) Current Opinion in Immunology 11:340.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFRSF18