GIT1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP1-31447] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-31447] - Paraffin-embedded mixed OVCA, using antibody at 1:100 dilution.

Western Blot: GIT1 Antibody [NBP1-31447] - Rat2 whole cell lysate/extract.

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GIT1 antibody [N3C2], Internal (NBP1-31447) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GIT1 antibody [N3C2], Internal (NBP1-31447) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GIT1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GIT1 Antibody - BSA Free (NBP1-31447) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-GIT1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GIT1. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GIT1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 84 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for GIT1 Antibody - BSA Free

• ARF GAP GIT1
• ARF GTPase-activating protein GIT1
• CAT1
• CAT-1
• Cool-associated and tyrosine-phosphorylated protein 1
• G protein-coupled receptor kinase interacting ArfGAP 1
• G protein-coupled receptor kinase interactor 1
• G protein-coupled receptor kinase-interactor 1
• GIT1
• GRK-interacting protein 1

Background

G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules. Alternative names for GIT1 include GRK-interacting protein 1, cool-associated and tyrosine phosphorylated protein 1, and Cat-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for GIT1 Antibody (NBP1-31447)(2)

Publications using NBP1-31447

• Nagahori K, Kuramasu M, Kawata S et al. GIT1 is an untolerized autoantigen involved in immunologic disturbance of spermatogenesis Histochemistry and cell biology 2022-01-23 [PMID: 35066604] (WB, Mouse)
• Boczonadi V, Gillespie R, Keenan I et al. Scrib:Rac1 interactions are required for the morphogenesis of the ventricular myocardium Cardiovasc. Res. 2014-08-18 [PMID: 25139745] (WB, Mouse, Rat)

Bioinformatics

• Gene Symbol: GIT1