GIT2 Antibody

Images

 
Western Blot: GIT2 Antibody [NBP2-16661] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GIT2 antibody [N1N3] diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: GIT2 Antibody [NBP2-16661] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GIT2 protein stained by GIT2 antibody [N1N3] diluted at 1:500. Blue: Hoechst ...read more
Western Blot: GIT2 Antibody [NBP2-16661] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GIT2 antibody [N1N3] diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, RM, XpSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.81 mg/ml

Order Details

GIT2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human GIT2. The exact sequence is proprietary.
Predicted Species
Rat (97%), Chicken (96%), Xenopus (93%), Rhesus Macaque (99%), Bovine (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GIT2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (93%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.81 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GIT2 Antibody

  • ARF GAP GIT2
  • ARF GTPase-activating protein GIT2
  • CAT2
  • CAT-2
  • cool-associated, tyrosine phosphorylated protein 2
  • Cool-interacting tyrosine-phosphorylated protein 2
  • DKFZp686G01261
  • G protein-coupled receptor kinase interacting ArfGAP 2
  • G protein-coupled receptor kinase interactor 2
  • G protein-coupled receptor kinase-interactor 2
  • KIAA0148GRK-interacting protein 2
  • MGC760

Background

This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GIT2
Uniprot