GIT1 Antibody

Western Blot: GIT1 Antibody [NBP1-19141] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NBP1-19141 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. GIT1 was also immunoprecipitated by NBP1-19142, which recognizes a downstream epitope.

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-19141] - Human breast carcinoma. Antibody used at a dilution of 1:200.

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-19141] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-GIT1 used at a dilution of 1:250.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

GIT1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 375 and 425 of human G protein-coupled receptor kinase interacting ArfGAP 1 using the numbering given in entry NP_054749.2 (GeneID 28964).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GIT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GIT1 Antibody

• ARF GAP GIT1
• ARF GTPase-activating protein GIT1
• CAT1
• CAT-1
• Cool-associated and tyrosine-phosphorylated protein 1
• G protein-coupled receptor kinase interacting ArfGAP 1
• G protein-coupled receptor kinase interactor 1
• G protein-coupled receptor kinase-interactor 1
• GIT1
• GRK-interacting protein 1

Background

G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules. Alternative names for GIT1 include GRK-interacting protein 1, cool-associated and tyrosine phosphorylated protein 1, and Cat-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GIT1
• Entrez:
  • 28964(Human)
• Uniprot:
  • NP_054749.2(Human)