GIT1 Antibody

Images

 
Western Blot: GIT1 Antibody [NBP1-19141] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NBP1-19141 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and ...read more
Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-19141] - Human breast carcinoma. Antibody used at a dilution of 1:200.
Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-19141] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-GIT1 used at a dilution of 1:250.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

GIT1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 375 and 425 of human G protein-coupled receptor kinase interacting ArfGAP 1 using the numbering given in entry NP_054749.2 (GeneID 28964).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GIT1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GIT1 Antibody

  • ARF GAP GIT1
  • ARF GTPase-activating protein GIT1
  • CAT1
  • CAT-1
  • Cool-associated and tyrosine-phosphorylated protein 1
  • G protein-coupled receptor kinase interacting ArfGAP 1
  • G protein-coupled receptor kinase interactor 1
  • G protein-coupled receptor kinase-interactor 1
  • GIT1
  • GRK-interacting protein 1

Background

G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules. Alternative names for GIT1 include GRK-interacting protein 1, cool-associated and tyrosine phosphorylated protein 1, and Cat-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GIT1
Entrez
Uniprot