GIT1 Antibody (S39B-8) [Alexa Fluor™ Plus 680]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP, KO
• Clone: S39B-8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 680

GIT1 Antibody (S39B-8) [Alexa Fluor™ Plus 680] Summary

• Immunogen: Fusion protein amino acids 375-770 (C-terminus) of rat GIT1
• Localization: Cytoplasm
• Specificity: Detects approx 90kDa. Does not cross-react with GIT2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GIT1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30546041).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for GIT1 Antibody (S39B-8) [Alexa Fluor™ Plus 680]

• ARF GAP GIT1
• ARF GTPase-activating protein GIT1
• CAT1
• CAT-1
• Cool-associated and tyrosine-phosphorylated protein 1
• G protein-coupled receptor kinase interacting ArfGAP 1
• G protein-coupled receptor kinase interactor 1
• G protein-coupled receptor kinase-interactor 1
• GIT1
• GRK-interacting protein 1

Background

G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules. Alternative names for GIT1 include GRK-interacting protein 1, cool-associated and tyrosine phosphorylated protein 1, and Cat-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GIT1