GIPR Antibody - BSA Free

Immunohistochemistry-Paraffin: GIPR Antibody [NLS1251] - Analysis of anti-GIPR antibody with human pancreas.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GIPR Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 19 amino acid peptide from N-terminal extracellular domain of human GIPR.
• Epitope: N-Terminus extracellular domain
• Localization: Integral membrane protein.
• Specificity: Human GIPR. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GHRHR (100%).
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GIPR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 8 - 21 ug/ml
• Application Notes: Use in Western blot reported in scientific literature (PMID: 26041107)..

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (95%), Canine (89%), Hamster (89%), Rabbit (84%). Rat reactivity reported in scientific literature (PMID: 26041107). Mouse reactivity reported in scientific literature (PMID: 29275960).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GIPR Antibody - BSA Free

• gastric inhibitory polypeptide receptor
• GIPR
• GIP-R
• Glucose-dependent insulinotropic polypeptide receptor
• MGC126722
• PGQTL2

Background

GIPR, or Gastric Inhibitory Polypeptide Receptor, mediates GIP-induced secretion of insulin by pancreatic islet beta cells after a meal. GIP is a gastrointestinal peptide hormone of 42 aa that is released from duodenal endocrine K cells after absorption of glucose or fat. Stimulation of the GIPR on pancreatic cells activates adenylyl cyclase and mitogen-activated protein kinase, resulting in increased insulin secretion. Mice with a targeted mutation of GIPR have higher blood glucose levels with impaired initial insulin response after oral glucose load. Analysis of GIPR knockout mice suggest that GIPR defects may contribute to the pathogenesis of diabetes and obesity. Ectopic expression of functional GIPR and its coupling to steroidogenesis has been suggested to be the main cause of food-dependent Cushing's syndrome. Two isoforms of GIPR are produced by alternative splicing. GIPR expression has been reported in human bone, fetal adrenal, and pancreas. Little expression has been identified in normal adult adrenal, but overexpression of GIPR has been observed in the adrenal in food-dependent Cushing's syndrome. GIPR expression has been identified in rat brain, heart, pancreas, and small intestine. ESTs have been isolated from colon libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GIPR Antibody (NLS1251)(4)

Publications using NLS1251

• Ussher JR, Campbell JE, Mulvihill EE et al. Inactivation of the Glucose-Dependent Insulinotropic Polypeptide Receptor Improves Outcomes following Experimental Myocardial Infarction. Cell Metab. 2017-12-20 [PMID: 29275960] (Mouse)
• Snook LA, Nelson EM, Dyck DJ et al. Glucose-dependent insulinotropic polypeptide directly induces glucose transport in rat skeletal muscle Am. J. Physiol. Regul. Integr. Comp. Physiol. 2015 Jun 03 [PMID: 26041107] (WB, Rat)
• Gao L, Coope H, Grant S et al. ABIN1 protein cooperates with TAX1BP1 and A20 proteins to inhibit antiviral signaling. J Biol Chem 2011-10-21 [PMID: 21885437]
• Kim SJ, Nian C, McIntosh CH. Adipocyte expression of the glucose-dependent insulinotropic polypeptide receptor involves gene regulation by PPARgamma and histone acetylation. J Lipid Res 2011-04-01 [PMID: 21245029] (Mouse)

Bioinformatics

• Gene Symbol: GIPR
• Entrez:
  • 2696(Human)
  • 25024(Rat)
• Uniprot:
  • P48546()