Recombinant Human GILT/IFI30 His Protein

Images

 
SDS-Page: Recombinant Human GILT/IFI30 Protein [NBP2-23146] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. Molecular Weight: 22.5 kDa (199aa) confirmed by MALDI-TOF

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human GILT/IFI30 His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 58-232 of Human GILT/IFI30

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNAPLVN VTLYYEALCG GCRAFLIREL FPTWLLVMEI LNVTLVPYGN AQEQNVSGRW EFKCQHGEEE CKFNKVEACV LDELDMELAF LTIVCMEEFE DMERSLPLCL QLYAPGLSPD TIMECAMGDR GMQLMHANAQ RTDALQPPHE YVPWVTVNGK PLEDQTQLLT LVCQLYQGK

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
IFI30
Purity
>85%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 10% glycerol, 1 mM DTT
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>85%, by SDS-PAGE

Alternate Names for Recombinant Human GILT/IFI30 His Protein

  • EC 1.8
  • Gamma-interferon-inducible protein IP-30
  • GILT
  • GILTgamma-interferon-inducible lysosomal thiol reductase
  • IFI30
  • IFI-30
  • interferon, gamma-inducible protein 30
  • IP30
  • IP30interferon gamma-inducible protein 30 preproprotein
  • Legumaturain
  • MGC32056

Background

IFI30, also as known as gamma-interferon-inducible lysosomal thiol reductase, belongs to the GILT family. This protein is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. This protein facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or cross-presentation. Recombinant human IFI30 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IFI30