Ghrelin/Obestatin Antibody

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Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Ghrelin/Obestatin Antibody Summary

Immunogen
GSS(n Octanoyl)FLSPEHQKAQQ, corresponding to amino acids 24-37 of Ghrelin.
Epitope
N14 region of Ghrelin.
Localization
Secreted
Specificity
Ghrelin
Clonality
Polyclonal
Host
Chicken
Gene
GHRL
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • ELISA 5 ug/ml
Application Notes
WB: Predicted molecular weight: 13 kDa. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ghrelin/Obestatin Antibody

  • appetite-regulating hormone
  • ghrelin
  • ghrelin/obestatin preprohormone
  • ghrelin/obestatin prepropeptide
  • Ghrelin/Obestatin
  • Growth hormone secretagogue
  • Growth hormone-releasing peptide
  • Motilin-related peptide
  • MTLRPgrowth hormone secretagogue receptor ligand
  • obestatin
  • Protein M46

Background

Ghrelin was originally discovered as a preprohormone that stimulates the release of growth hormone from the anterior pituitary. Recent studies have shown that ghrelin, in concert with other hormones, helps to control both appetite and energy balance. The synthesis of ghrelin takes place predominantly in epithelial cells of the stomach, although it is also produced in smaller quantities in the placenta, kidney, pituitary and hypothalamus. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release from the pituitary. It has an appetite stimulating effect, induces adiposity, and stimulates gastric acid secretion, and it is involved in growth regulation. Ghrelin is synthesized as a preprohormone (13kDa), then proteolytically processed to yield a 28 amino acid peptide (3kDa). Obestatin, an appetite suppressing peptide hormone, is also produced in the process. An interesting and unique modification is imposed on the ghrelin hormone during synthesis in the form of octanoic acid bound to one of its amino acids. This modification is necessary for biological activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GHRL
Entrez