Ghrelin/Obestatin Antibody (121) - BSA Free

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
121
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Ghrelin/Obestatin Antibody (121) - BSA Free Summary

Immunogen
Synthetic ghrelin (14-28)
Specificity
It binds human ghrelin in solution.
Isotype
IgG2 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GHRL
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:4000
Application Notes
This product is useful for ELISA and WB/Immunoblot. The ELISA dilution guideline of 1/4000 applies to synthetic human ghrelin coated directly at 1.0 microgram/milliliter

Reactivity Notes

It binds human ghrelin and cross-reacts with rat ghrelin. It is presumed to cross-react with ghrelins of other species with identical 14-28 sequences, such as mouse, dog and cat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Phosphate (pH 7.4) and 0.5M NaCl
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Ghrelin/Obestatin Antibody (121) - BSA Free

  • appetite-regulating hormone
  • ghrelin
  • ghrelin/obestatin preprohormone
  • ghrelin/obestatin prepropeptide
  • Ghrelin/Obestatin
  • Growth hormone secretagogue
  • Growth hormone-releasing peptide
  • Motilin-related peptide
  • MTLRPgrowth hormone secretagogue receptor ligand
  • obestatin
  • Protein M46

Background

Ghrelin is an orexigenic (food intake stimulating) 27 or 28 amino-acid gut-brain peptide hormone produced by endocrine cells in the stomach, pancreatic islets and certain hypothalamic neurons1,2. Two forms are produced by alternative mRNA splicing; Gln13 or 14 is missing from the second form."Active" ghrelin is acylated (octanoylated) at Ser3; this form stimulates growth hormone secretion via GHS receptors and increases appetite. The des-acyl form does not have these effects but may have other actions. Plasma responses of total ghrelin to food reflect those of active ghrelin3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-47182B

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Bioinformatics Tool for Ghrelin/Obestatin Antibody (NBP1-47182)

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Bioinformatics

Gene Symbol GHRL
Entrez