GFR alpha-3/GDNF R alpha-3 Antibody [Unconjugated]

Western blot shows lysates of human stomach tissue and human small intestine tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human GFRa-3/GDNF Ra-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF670) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for GFRa-3/GDNF Ra-3 at approximately 55 and 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

GFR alpha-3/GDNF R alpha-3 Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human GFR alpha ‑3/GDNF R alpha ‑3
  Met1-Trp382
  Accession # AAC24355
• Specificity: Detects human GFR alpha ‑3/GDNF R alpha ‑3 in direct ELISAs and Western blots. In direct ELISA and Western blots, no cross-reactivity with recombinant human GFR alpha -2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GFRA3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFR alpha-3/GDNF R alpha-3 Antibody [Unconjugated]

• GDNF family receptor alpha 3
• GDNF family receptor alpha-3
• GDNF R alpha-3
• GDNF receptor alpha-3
• GDNFR-alpha-3
• GFR alpha3
• GFR alpha-3
• GFRA3
• GFRa-3
• GFR-alpha-3
• glial cell line-derived neurotrophic factor receptor alpha-3
• GPI-linked receptor

Background

Glial cell line-derived growth factor (GDNF), neurturin (NTN), persephin (PEP) and artemin (ART), distant members of the TGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of these neurotrophic factors are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and one of four ligand binding cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins (GFR alpha -1, 2, 3 or 4). These four GFR-alpha  proteins share conserved placements of many of their cysteine residues. GFR alpha -1, 2, 3 or 4 have been shown to preferentially bind GDNF, NTN, ART and PSP, respectively. While the GFR alpha -3/Ret complex is highly specific for ART, the GFR alpha -1 /Ret complex is extremely promiscuous and may also be utilized by NTN and ART.

Human GFR alpha -3 cDNA encodes a 400 amino acid (aa) residue precursor protein with an N-terminal signal peptide and C-terminal hydrophobic domain. GFR alpha -3 is approximately 34% and 36% identical to GFR alpha -1 and GFR alpha -2, respectively. Human and mouse GFR alpha -3 share approximately 76% amino acid sequence homology. The gene encoding GFR alpha -3 has been localized to human chromosome 5. GFR alpha -3 is expressed at high levels only in the developing and adult sensory and sympathetic ganglia of the peripheral nervous system. In the trigeminal ganglions, the expression of GFR alpha -3 is found in a population of neurons distinct from those expressing GFR alpha -1 or 2.

1. Baloh, R. et al. (1998) Neuron 21:1291.
2. Baloh, R. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
3. Worby, C. et al. (1998) J. Biol. Chem. 273:3502.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFR alpha-3/GDNF R alpha-3 Antibody (AF670)(2)

Publications using AF670

• Iwasaki, T;Akeda, K;Kawaguchi, K;Yamada, J;Hasegawa, T;Takegami, N;Fujiwara, T;Sudo, A; Expression of Glial-Cell-Line-Derived Neurotrophic Factor Family Ligands in Human Intervertebral Discs International journal of molecular sciences 2023-11-01 [PMID: 37958856] (Western Blot, Human)
• Negri T, Virdis E, Brich S, Bozzi F, Tamborini E, Tarantino E, Jocolle G, Cassinelli G, Grosso F, Sanfilippo R, Casalini P, Greco A, Pierotti MA, Pilotti S Functional mapping of receptor tyrosine kinases in myxoid liposarcoma. Clin. Cancer Res., 2010-06-03;16(14):3581-93. 2010-06-03 [PMID: 20522586] (IHC-P, Western Blot, Human)

Reviews for GFR alpha-3/GDNF R alpha-3 Antibody (AF670) (2)

Average Rating: 3

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer IP Human 12/19/2014

Summary

• Application: Immunoprecipitation
• Sample Tested: HEK293 cells transfected with GFR alpha-3
• Species: Human

reviewed by: Verified Customer WB Human 12/19/2014

Summary

• Application: Western Blot
• Sample Tested: HEK293 cells transfected with GFR alpha-3
• Species: Human

Bioinformatics

• Gene Symbol: GFRA3
• Uniprot:
  • AAC24355()