GFR alpha-2/GDNF R alpha-2 Antibody [Alexa Fluor® 700]

• Reactivity: Hu, Mu
• Applications: WB, IHC, B/N, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 700

GFR alpha-2/GDNF R alpha-2 Antibody [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse GFR alpha ‑2/GDNF R alpha ‑2
  Ser22-Ser441
  Accession # Q3UUD8
• Specificity: Detects human and mouse GFR alpha ‑2/GDNF R alpha ‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
  5% cross‑reactivity with recombinant human GFR alpha -3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFR alpha-2/GDNF R alpha-2 Antibody [Alexa Fluor® 700]

• GDNF family receptor alpha 2
• GDNF family receptor alpha-2
• GDNF R alpha-2
• GDNF receptor alpha-2
• GDNF receptor beta
• GDNFR-alpha-2
• GDNFR-beta
• GDNFRBRET ligand 2
• GFR alpha2
• GFR alpha-2
• GFRA2
• GFRa-2
• GFR-alpha 2
• GFR-alpha-2
• glial cell line derived neurotrophic factor receptor, beta
• Neurturin receptor alpha
• NRTNR-alpha
• NTNRA
• NTNR-alpha
• PI-linked cell-surface accessory protein
• RETL2TGF-beta-related neurotrophic factor receptor 2
• TGF-beta related neurotrophic factor receptor 2
• TRN receptor, GPI-anchored
• TRNR2NRTNR-ALPHA

Background

Glial cell line-derived growth factor (GDNF), neurturin (NTN), persephin (PSP) and artemin, distant members of the TGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits, GDNF receptor alpha -1 (GFR alpha -1, also known as Trn R1) or GFR alpha -2 (also known as Trn R2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF or NTN to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase.

Mouse GFR alpha -2 cDNA encodes a 463 amino acid (aa) residue protein with a putative N-terminal 21 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -2 has a C-terminal hydrophobic region which is preceded by a 3 aa residue (SGS) GPI-binding site. Human GFR alpha -2 shares 96.5% amino acid identity with mouse GFR alpha -2. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q3UUD8()