GFR alpha-1/GDNF R alpha-1 Antibody (260714) [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clone: 260714
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

GFR alpha-1/GDNF R alpha-1 Antibody (260714) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human GFR alpha -1
  Asp25-Lys434
  Accession # P56159
• Specificity: Detects human GFR alpha -1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human GFR alpha -2, -3, -4, or recombinant rat GFR alpha -1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GFRA1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GFR alpha-1/GDNF R alpha-1 Antibody (260714) [Unconjugated]

• DKFZp313E1012
• DKFZp686J0156
• GDNF family receptor alpha 1
• GDNF family receptor alpha-1
• GDNF R alpha-1
• GDNF receptor alpha-1
• GDNFRAGFR-ALPHA-1
• GDNFR-alpha-1
• GDNFRMGC23045
• GFR alpha1
• GFR alpha-1
• GFRA1
• GFRa-1
• GFR-alpha-1
• Glial cell line-derived neurotrophic factor receptor alpha
• GPI-linked anchor protein
• PI-linked cell-surface accessory protein
• RET ligand 1
• RET1L
• RETL1FLJ10561
• TGF-beta related neurotrophic factor receptor 1
• TGF-beta-related neurotrophic factor receptor 1
• TRNR1FLJ31546

Background

Glial cell line-derived growth factor (GDNF), neurturin (NTN), artemin and persephin are distant members of the TGF-beta superfamily. They function as neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits [GDNF receptor alpha -1 (GFR alpha -1) or GFR alpha -2]. GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase. Human GFR alpha -1 cDNA encodes a 465 amino acid (aa) residue protein with an N-terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, human GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% aa identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFR alpha-1/GDNF R alpha-1 Antibody (MAB7141)(3)

Publications using MAB7141

• Mahmoud Huleihel, Seyedmehdi Nourashrafeddin, Seyedmehdi Nourashrafeddin Application of three-dimensional culture systems to study mammalian spermatogenesis, with an emphasis on the rhesus monkey (Macaca mulatta) Asian Journal of Andrology 2015-06-13 [PMID: 26067870]
• Brian P. Hermann, Meena Sukhwani, David R. Simorangkir, Tianjiao Chu, Tony M. Plant, Kyle E. Orwig Molecular dissection of the male germ cell lineage identifies putative spermatogonial stem cells in rhesus macaques Human Reproduction 2009-07-01 [PMID: 19336441]
• Hermann BP, Sukhwani M, Lin CC, Sheng Y, Tomko J, Rodriguez M, Shuttleworth JJ, McFarland D, Hobbs RM, Pandolfi PP, Schatten GP, Orwig KE Characterization, cryopreservation, and ablation of spermatogonial stem cells in adult rhesus macaques. Stem Cells, 2007-06-21;25(9):2330-8. 2007-06-21 [PMID: 17585169] (IHC-P, Primate - Macaca mulatta (Rhesus Macaque))

Review for GFR alpha-1/GDNF R alpha-1 Antibody (MAB7141) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 02/25/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 22519471
• Species: Human

Bioinformatics

• Gene Symbol: GFRA1
• Uniprot:
  • P56159()