Geminin Antibody

Images

 
Immunohistochemistry: Geminin Antibody [NB100-40826] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Geminin used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: Geminin Antibody [NB100-40826] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-Geminin used at a dilution of 1:250.
Immunoprecipitation: Geminin Antibody [NB100-40826] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-Geminin antibody NB100-40826 used for IP at 3 mcg/mg lysate. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC-P, IP, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Geminin Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human Geminin DNA replication Inhibitor using the numbering given in entry NP_056979.1 (GeneID 51053).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GMNN
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1:2000-1:10000
  • Immunoprecipitation 2-5 ug/mg
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Positive Control
Geminin Lysate (NBL1-11143)
Publications
Read Publication using NB100-40826.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Geminin Antibody

  • Gem
  • geminin
  • geminin, DNA replication inhibitor
  • RP3-369A17.3

Background

Geminin inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex. It is degraded during the mitotic phase of the cell cycle, has a destruction box sequence (RRTLKVIQP), and is ubiquitinated by the anaphase promoting complex in vitro. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Further, geminin interacts tightly with CDT1, a replication initiation factor necessary for MCM loading.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Geminin Antibody (NB100-40826)(1)


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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GMNN
Entrez
Uniprot