Gemin 3 Antibody

Western Blot: Gemin 3 Antibody [H00011218-B01P] - Analysis of DDX20 expression in human placenta.

Immunocytochemistry/Immunofluorescence: Gemin 3 Antibody [H00011218-B01P] - Analysis of purified antibody to DDX20 on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: Gemin 3 Antibody [H00011218-B01P] - Analysis of DDX20 expression in transfected 293T cell line by DDX20 polyclonal antibody. Lane 1: DDX20 transfected lysate(90.64 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

Gemin 3 Antibody Summary

• Immunogen: DDX20 (AAH34953.1, 1 a.a. - 824 a.a.) full-length human protein. MAAAFEASGALAAVATAMPAEHVAVQVPAPEPTPGPVRILRTAQDLSSPRTRTGDVLLAEPADFESLLLSRPVLEGLRAAGFERPSPVQLKAIPLGRCGLDLIVQAKSGTGKTCVFSTIALDSLVLENLSTQILILAPTREIAVQIHSVITAIGIKMEGLECHVFIGGTPLSQDKTRLKKCHIAVGSPGRIKQLIELDYLNPGSIRLFILDEADKLLEEGSFQEQINWIYSSLPASKQMLAVSATYPEFLANALTKYMRDPTFVRLNSSDPSLIGLKQYYKVVNSYPLAHKVFEEKTQHLQELFSRIPFNQALVFSNLHSRAQHLADILSSKGFPAECISGNMNQNQRLDAMAKLKHFHCRVLISTDLTSRGIDAEKVNLVVNLDVPLDWETYMHRIGRAGRFGTLGLTVTYCCRGEEGNMMMRIAQKCNINLLPLPDPIPSGLMEECVDWDVEVKAAVHTYGIASVPNQPLKKQIQKIERTLQIQKAHGDHMASSRNNSVSGLSVKSKNNTKQKLPVKSHSECGIIEKATSPKELGCDRQSEEQMKNSVQTPVENSTNSQHQVKEALPVSLPQIPCLSSFKIHQPYTLTFAELVEDYEHYIKEGLEKPVEIIRHYTGPGDQTVNPQNGFVRNKVTEQRVPVLASSSQSGDSESDSDSYSSRTSSQSKGNKSYLEGSSDNQLKDSESTPVDDRISLEQPPNGSDTPNPEKYQESPGIQMKTRLKEGASQRAKQSRRNLPRRSSFRLQTEAQEDDWYDCHREIRLSFSDTYQDYEEYWRAYYRAWQEYYAAASHSYYWNAQRHPSWMAAYHMNTIYLQEMMHSNQ
• Specificity: Reacts with DEAD (Asp-Glu-Ala-Asp) box polypeptide 20.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: DDX20
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: This antibody is reactive against tissue and transfected lysate in western blot, immunofluorescence, and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Gemin 3 Antibody

• Component of gems 3
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
• DEAD box protein 20
• DEAD box protein DP 103
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD
• DKFZp434H052
• DP103DEAD-box protein DP103
• EC 3.6.4.13
• Gemin-3
• GEMIN3gemin-3
• probable ATP-dependent RNA helicase DDX20
• SMN-interacting protein

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX20
• Entrez:
  • 11218(Human)
• Uniprot:
  • AAH34953.1(Human)