GDF-9 Antibody (917319) [Alexa Fluor® 405] Summary
Human embryonic kidney cell line derived recombinant human GDF-9
Accession # O60383
Detects human GDF-9 in direct ELISAs.
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Flow Cytometry 0.25-1 ug/10^6 cells
Packaging, Storage & Formulations
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for GDF-9 Antibody (917319) [Alexa Fluor® 405]
- growth differentiation factor 9
- growth/differentiation factor 9
Growth Differentiation Factor-9 (GDF-9) is an oocyte secreted paracrine factor in the TGF-beta superfamily (1, 2). It is synthesized as a prepropeptide and is subsequently processed by proteases into the mature protein (1, 2). Mature human GDF-9 has a predicted molecular weight of 16 kDa and shares 89.6% and 91.9% amino acid sequence identity with the mouse and rat orthologs, respectively. Despite the high homology, mouse GDF-9 is secreted in an active form, while human GDF-9 is latent. A single mutation Gly391Arg increases the affinity between human GDF-9 and its signaling receptors and make it more active (3). It forms both non-covalent homodimers and heterodimers with BMP-15, which is coordinately expressed with GDF-9 in the oocyte. (2, 4, 5). GDF-9 signals through TGF-beta RI/ALK-5 and BMPR-II, while the GDF-9:BMP-15 heterodimer is believed to signal through BMPR-II, ALK 4/5/7, and BMPR-IB/ALK-6 (5-8). SMAD2 and SMAD3 are phosphorylated following activation of receptor complexes by GDF-9 (5, 6). GDF-9 functions as a paracrine factor in the development of primary follicles in the ovary. It is critical for the growth of granulosa and theca cells and for the differentiation and maturation of the oocyte (5, 9-11). GDF-9 is thought to act synergistically with BMP-15 to control development of the oocyte-cumulus cell complex (4-6). In humans, GDF-9:BMP-15 heterodimers have been shown to be more potent regulators of granulosa cell functions compared to GDF-9 homodimers (6). Aberrant GDF-9 expression and activation is associated with a multitude of common human ovarian disorders including premature ovarian failure and polycystic ovary syndrome (10, 12-14). In breast and bladder cancers, GDF-9 is believed to function as a tumor suppressor because its expression levels are inversely correlated with the aggressiveness of the cancer (15, 16). In prostate cancer, however, GDF-9 may enhance tumor progression by promoting tumor cell growth and epithelial-to-mesenchymal transition (17, 18).
- McGrath, S. A. et al. (1995) Mol. Endocrinol. 9:131.
- Aaltonen, J. et al. (1999) J. Clin. Endocrinol. Metab. 84:2744.
- Simpson, C.M. et al. (2012) 153:1301.
- Liao, W.X. et al. (2003) J. Biol. Chem. 278:3713.
- Gilchrist, R.B. et al. (2008) Hum. Reprod. Update 14:159.
- Peng, J. et al. (2013) Proc. Natl. Acad. Sci. USA 110:E776.
- Vitt, U.A. et al. (2002) Biol. Reprod. 67:473.
- Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653.
- Hreinsson, J.G. et al. (2002) J. Clin. Endocrinol. Metab. 87:316.
- Otsuka, F. et al. (2011) Mol. Reprod. Dev. 78:9.
- Dong, J. et al. (1996) Nature 383:531.
- Zhao, S.Y. et al. (2010) Fertil. Steril. 94:261.
- Wei, L.N. et al. (2011) Fertil. Steril. 96:464.
- Simpson, C.M. et al. (2014) J. Clin. Endocrinol. Metab. [Epub ahead of print].
- Hanavadi, S. et al. (2007) Ann. Surg. Oncol. 14:2159.
- Du, P. et al. (2012) Int. J. Mol. Med. 29:428.
- Bokobza, S.M. et al. (2010) J. Cell. Physiol. 225:529.
- Bokobza, S.M. et al. (2011) Mol. Cell. Biochem. 349:33.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Applications: WB, ELISA, ICC
Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ICC
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Species: Hu, Mu, Rt, Po, Bv, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready
Publications for GDF-9 Antibody (FAB8266V) (0)
There are no publications for GDF-9 Antibody (FAB8266V).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for GDF-9 Antibody (FAB8266V) (0)
There are no reviews for GDF-9 Antibody (FAB8266V).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for GDF-9 Antibody (FAB8266V) (0)
Other Available Formats
Additional GDF-9 Products
Blogs on GDF-9