GDF-3 Antibody (741614) [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 741614
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

GDF-3 Antibody (741614) [Alexa Fluor® 750] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human GDF-3
  Ala251-Gly364
  Accession # Q9NR23
• Specificity: Detects human GDF-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) GDF-1, rhBMP-2, or rhBMP-6 is observed and approximately 5% cross-reactivity with recombinant mouse GDF-3 homodimer is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDF-3 Antibody (741614) [Alexa Fluor® 750]

• GDF3
• GDF-3
• growth differentiation factor 3
• growth/differentiation factor 3
• KFS3
• MCOP7
• MCOPCB6
• Vgr-2

Background

GDF-3 (previously called Vgr-2) is a TGF-beta superfamily member belonging to the growth/differentiation factor family (1, 2). GDF-3 is expressed in undifferentiated embryonic stem (ES) cells, white adipose tissue and the brain (2-4). The 364 amino acid (aa) human GDF-3 contains a 21 aa signal sequence, a 229 aa propeptide and a 114 aa mature region that contains one potential N-glycosylation site. The mature region contains a cysteine-knot structure that is conserved throughout family members. However, it lacks the fourth cysteine which is responsible for the formation of an inter-molecular disulfide bond, so GDF-3 may exist as a non-covalent homodimer (2, 5). Mature human GDF-3 shares 83%, 83%, 91%, 92% and 93% aa identity with mouse, rat, bovine, canine and equine GDF-3, respectively. Most of GDF-3 is present as the uncleaved prepro form (6). The uncleaved and the mature forms both appear to have activity, but that activity may differ (5-8). All forms can oppose BMPs. In human ES cells, inhibition of BMP-2 signaling by GDF-3 maintains pluripotency (5, 7). GDF-3 also influences early cell fate decisions; for example, deletion of mouse GDF-3 produces defects in the anterior visceral endoderm of the pre-gastrulation embryo (6-8). GDF-3 cooperates with GDF-1 in embryogenesis, and the mature protein has nodal-like activity (8, 9). Although GDF family members signal through BMP receptors (ALK-1, -2, -3 and -6), which activate Smads 1, 5 and 8, GDF-3 signaling through ALK-4 and ALK-7, which activate Smads 2 and 3, has also been reported (9, 10). In adipocytes, GDF-3 is induced by a high fat diet, promoting adipogenesis and obesity (3, 10, 11).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.