GDF-1 Antibody (748450)

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Western blot shows lysates of human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GDF‑1 Monoclonal Antibody (Catalog # MAB6937) followed by HRP-conjugated Anti-Mouse IgG Secondary ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
748450
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

GDF-1 Antibody (748450) Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human GDF-1
Asp254-Arg372
Accession # NP_001483
Specificity
Detects human GDF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse (rm) GDF-1 is observed, and no cross-reactivity with recombinant human (rh) GDF-3 is observed. In Western blots, no cross-reactivity with rhGDF-3 or rmGDF-7 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
GDF1
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDF-1 Antibody (748450)

  • DORV
  • DTGA3
  • embryonic growth/differentiation factor 1
  • GDF1
  • GDF-1
  • growth differentiation factor 1

Background

Growth and Differentiation Factor 1 (GDF‑1) is a secreted protein in the TGF‑ beta superfamily (1). It is the functional equivalent of frog, zebrafish, and chick Vg1 (2, 3). Human GDF‑1 is synthesized as a preproprotein that produces an approximately 44 kDa proprotein after cleavage of a 29 amino acid (aa) signal peptide. Further proteolysis at a dibasic cleavage site produces a 224 aa propeptide and a secreted 119 aa, 12‑15 kDa mature peptide (4). Mature human GDF‑1 shares 81% aa sequence identity with mouse and rat GDF‑1. GDF‑1 is expressed in the developing and adult nervous system, particularly in neurons of the cerebral cortex and hippocampus (4, 5). It is required during embryogenesis for development of left/right axis asymmetry, anterior/posterior axis asymmetry, and the notochord (2, 3, 6, 7). It cooperates with Nodal and GDF‑3 in these activities and potentiates Nodal bioactivity through formation of a 27 kDa noncovalent GDF‑1‑Nodal heterodimer (3, 7, 8). The presence of Nodal is required for GDF‑1 induced effects (8). GDF‑1 signals through heteromeric receptor complexes containing Activin RIIA or Activin RIIB, ALK4 or ALK7, and an EGF‑CFC protein such as Cryptic or Cripto (7‑9). Loss of function mutations in GDF‑1 are associated with a variety of human cardiac development defects (10).
  1. Rider, C.C. and B. Mulloy (2010) Biochem. J. 429:1.
  2. Wall, N.A. et al. (2000) Dev. Biol. 227:495.
  3. Andersson, O. et al. (2007) Dev. Biol. 311:500.
  4. Lee, S.-J. (1991) Proc. Natl. Acad. Sci. 88:4250.
  5. Soderstrom, S. and T. Ebendal (1999) J. Neurosci. Res. 56:482.
  6. Rankin, C.T. et al. (2000) Nat. Genet. 24:262.
  7. Andersson, O. et al. (2006) Dev. Biol. 293:370.
  8. Tanaka, C. et al. (2007) Genes Dev. 21:3272.
  9. Cheng, S.K. et al. (2003) Genes Dev. 17:31.
  10. Karkera, J.D. et al. (2007) Am. J. Hum. Genet. 81:987.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GDF1
Entrez
Uniprot