Western Blot: GDF-15 Antibody [Biotin] [NBP1-42720] - Analysis shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were ...read more
Immunohistochemistry: GDF-15 Antibody [Biotin] [NBP1-42720] - Analysis of (C-terminal specific) at 20X in colon tissue at pH 6. Negative control of human colon tissue pH6 is shown in background. Tissue: Human Colon
Western Blot: GDF-15 Antibody [Biotin] [NBP1-42720] - Anti-NAG-1 (D variant) (MOUSE) Monoclonal Antibody. The blot shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - ...read more
Immunohistochemistry-Paraffin: GDF-15 Antibody [Biotin] [NBP1-42720] - GDF15 in mouse choroid plexus. Image from verified customer review.
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GDF-15 Antibody [Biotin] Summary
This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH.
This antibody specifically reacts with the amino terminal end of human NAG-1 protein from human tissues. A BLAST analysis was used to suggest partial reactivity.
Immunogen affinity purified
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This affinity purified antibody is suitable for ELISA and western blotting of human and mouse NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15, H variant or D variant specific antibodies. Expect bands in Western blots of approximately 14 and 28 kDa in size corresponding - NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract. Although not confirmed, this antibody may work in IHC.
Reactive in human. 92% identity to Chimpanzee and Macaque.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01% Sodium Azide
Immunogen affinity purified
Reconstitute with 50 ul of distilled water to a final concentration of 1.0 mg/ml.
Alternate Names for GDF-15 Antibody [Biotin]
growth differentiation factor 15
growth/differentiation factor 15
Macrophage inhibitory cytokine 1
MIC-1NSAID-activated gene 1 protein
MIC1Prostate differentiation factor
NAG-1NSAID-regulated gene 1 protein
NSAID (nonsteroidal inflammatory drug)-activated protein 1
Placental bone morphogenetic protein
Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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