GDF-15 Antibodies

957-GD) was serially diluted and captured by Mouse Anti-Human/Primate GDF‑15 Monoclonal Antibody (Catalog # MAB957) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human GDF‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF957) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 957-GD) was serially diluted and captured by Mouse Anti-Human/Primate GDF‑15 Monoclonal Antibody (Catalog # MAB957) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human GDF‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF957) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

HAF017). A specific band was detected for GDF-15 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for GDF-15 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysate of HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human GDF-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF957) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GDF-15 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

957-GD) was serially diluted and captured by Mouse Anti-Human/Primate GDF‑15 Monoclonal Antibody (Catalog # MAB957) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human GDF‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF957) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 957-GD) was serially diluted and captured by Mouse Anti-Human/Primate GDF‑15 Monoclonal Antibody (Catalog # MAB957) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human GDF‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF957) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="GDF-15 was detected in perfusion fixed frozen sections of mouse brain (trigeminal ganglion) using Sheep Anti-Mouse GDF-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6385) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="GDF-15 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human GDF-15 Monoclonal Antibody (Catalog # MAB9572) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="GDF-15 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining) and U937 human histiocytic lymphoma cell line (negative staining) using Mouse Anti-Human GDF-15 Monoclonal Antibody (Catalog # MAB9573) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="GDF-15 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive) and U937 human histiocytic lymphoma cell line (negative control) using Mouse Anti-Human GDF-15 Monoclonal Antibody (Catalog # MAB9571) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />