GDF-15 Antibody [Unconjugated]

GDF-15 was detected in perfusion fixed frozen sections of mouse brain (trigeminal ganglion) using Sheep Anti-Mouse GDF-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6385) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

GDF-15 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse GDF-15
  Ser189-Ala303
  Accession # Q9Z0J7
• Specificity: Detects mouse GDF-15 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: GDF15
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDF-15 Antibody [Unconjugated]

• GDF15
• GDF-15
• growth differentiation factor 15
• growth/differentiation factor 15
• Macrophage inhibitory cytokine 1
• MIC-1
• MIC-1NSAID-activated gene 1 protein
• MIC1Prostate differentiation factor
• NAG-1
• NAG-1NSAID-regulated gene 1 protein
• NSAID (nonsteroidal inflammatory drug)-activated protein 1
• PDF
• PDFGDF-15
• PLAB
• PLABNRG-1
• Placental bone morphogenetic protein
• Placental TGF-beta
• PTGF-beta
• PTGFBPTGF-beta

Background

GDF-15 (Growth/differentiation factor 15; also PTGF, PDF, PL-74 and NAG-1) is a 25-30 kDa homodimeric member of the TGF-beta superfamily of proteins. In rodent, GDF-15 is expressed in a diverse population of activated cell types, including hepatocytes, macrophages, Schwann cells, cardiomyocytes, osteoblasts, adipocytes, and epithelium of the small intestine and mammary gland. Functionally, GDF-15 has multiple effects, some tissue specific, including the induction of ACRP30 secretion from fat, an attenuation of the adverse effects of cardiac hypertrophy, and serving as a trophic factor for motor and sensory neurons. Mouse GDF-15 is synthesized as a 273 amino acid (aa) proprecursor that contains a cleavable 26 kDa, 158 aa glycosylated prodomain (aa 31-188) and a 12-14 kDa, 115 aa mature region (aa 189-303). In human, certain cells are noted to secrete an uncleaved 40 kDa proprecursor that, as a disulfide-linked homodimer, would run at about 80 kDa in nonreducing SDS-PAGE. Over aa 189-303, mouse GDF-15 shares 97% and 67% aa identity with rat and human GDF-15, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GDF-15 Antibody (AF6385)(3)

Publications using AF6385

• Sakie Katsumura, Nadeem Siddiqui, Michael Rock Goldsmith, Jaime H. Cheah, Teppei Fujikawa, Genki Minegishi et al. Deadenylase-dependent mRNA decay of GDF15 and FGF21 orchestrates food intake and energy expenditure Cell Metabolism 2022-04-05 [PMID: 35385705]
• H Ozawa, H Imazeki, Y Ogiwara, H Kawakubo, K Fukuda, Y Kitagawa, C Kudo-Saito Targeting AURKA in treatment of peritoneal tumor dissemination in gastrointestinal cancer Translational Oncology, 2021-12-10;16(0):101307. 2021-12-10 [PMID: 34902741] (Flow Cytometry, Mouse)
• E Fung, L Kang, D Sapashnik, S Benard, A Sievers, Y Liu, G Yan, J Zhou, L Rodriguez, W Ma, WR Stochaj, E LaVallie, L Wroblewska, K Kelleher, A Tam, O Bezy, D Breen, JR Chabot, T He, L Lin, Z Wu, L Mosyak Fc-GDF15 glyco-engineering and receptor binding affinity optimization for body weight regulation Scientific Reports, 2021-04-26;11(1):8921. 2021-04-26 [PMID: 33903632] (ELISA Capture, Mouse)

Bioinformatics

• Gene Symbol: GDF15
• Uniprot:
  • Q9Z0J7()