GDF-15 Antibody Pair [HRP]

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Sandwich ELISA: GDF-15 Antibody Pair [HRP] [NBP2-79325] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, S-ELISA
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP
Standard Curve Range
4.69-300 pg/ml (example only; lot dependent)
Sensitivity
4.69 pg/ml (example only; lot dependent)

Order Details

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Catalog# & Conjugate Size Price

GDF-15 Antibody Pair [HRP] Summary

Description
Solid Phase sandwich ELISA for the quantitative determination of Human GDF-15.
Specificity
This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human GDF-15.
Standard Curve Range
4.69-300 pg/ml (example only; lot dependent)
Sensitivity
4.69 pg/ml (example only; lot dependent)
Assay Type
Sandwich ELISA
Sample Volume
100 ul
Clonality
Monoclonal
Host
Mouse
Gene
GDF15

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
Application Notes
1. Capture Antibody: Dilute to a working concentration of 2 ug/ml in PBS before coating.
2. Detection Antibody: Dilute to working concentration of 0.15 ug/ml in detection antibody dilution buffer before use.
3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 300 pg/ml is recommended.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Capture Antibody: 1 mg/ml of mouse anti-Human GDF-15 monoclonal antibody (in PBS, pH 7.4).
  2. Detection Antibody: 0.2 mg/ml of mouse anti-Human GDF-15 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  3. Standard: Each vial contains 15 ng of recombinant Human GDF-15.

Alternate Names for GDF-15 Antibody Pair [HRP]

  • GDF15
  • GDF-15
  • growth differentiation factor 15
  • growth/differentiation factor 15
  • Macrophage inhibitory cytokine 1
  • MIC-1
  • MIC-1NSAID-activated gene 1 protein
  • MIC1Prostate differentiation factor
  • NAG-1
  • NAG-1NSAID-regulated gene 1 protein
  • NSAID (nonsteroidal inflammatory drug)-activated protein 1
  • PDF
  • PDFGDF-15
  • PLAB
  • PLABNRG-1
  • Placental bone morphogenetic protein
  • Placental TGF-beta
  • PTGF-beta
  • PTGFBPTGF-beta

Background

Growth differentiation factor 15 (GDF15) is a transforming growth factor beta superfamily member that acts to regulate inflammatory and apoptotic responses in injured or diseased tissues. GDF15 is most strongly expressed in the liver, placenta and kidney.

GDF15 antibodies are useful for studies on inflamation and immune system research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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Blogs on GDF-15.

Taking Biomarker Discovery From 2D to 3D: Increased Biological Activity of EVs Isolated From 3D Prostate Cancer Cultures
Jamshed Arslan, Pharm D, PhD Tissues within the human body are made of a three-dimensional (3D) arrangement of cells working together to perform vital functions. The commonly used 2D monolayer cultures have limited ...  Read full blog post.

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Bioinformatics

Gene Symbol GDF15