GDF-15 Antibody (147640) [Unconjugated]

GDF-15 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive) and U937 human histiocytic lymphoma cell line (negative control) using Mouse Anti-Human GDF-15 Monoclonal Antibody (Catalog # MAB9571) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 147640
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GDF-15 Antibody (147640) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human GDF-15
  Ala197-Ile308
  Accession # Q99988
• Specificity: Detects human GDF-15 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDF-15 Antibody (147640) [Unconjugated]

• GDF15
• GDF-15
• growth differentiation factor 15
• growth/differentiation factor 15
• Macrophage inhibitory cytokine 1
• MIC-1
• MIC-1NSAID-activated gene 1 protein
• MIC1Prostate differentiation factor
• NAG-1
• NAG-1NSAID-regulated gene 1 protein
• NSAID (nonsteroidal inflammatory drug)-activated protein 1
• PDF
• PDFGDF-15
• PLAB
• PLABNRG-1
• Placental bone morphogenetic protein
• Placental TGF-beta
• PTGF-beta
• PTGFBPTGF-beta

Background

Growth Differentiation Factor 15 (GDF-15), also called Macrophage inhibitory cytokine-1 (MIC-1), placental transforming growth factor-beta , prostate-derived factor, and placental bone morphogenetic protein, is a divergent member of the transforming growth factor beta (TGF-beta ) superfamily. GDF-15 is highly expressed in placenta and is expressed at lower levels in kidney, pancreas, prostate and colon. It is also widely expressed in brain. Similarly to other TGF-beta family proteins, GDF-15 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of GDF-15 contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Furthermore, the carboxy-terminal domain contains two additional cysteine residues that form a fourth intrachain disulfide bond. Biologically active GDF-15 is a disulfide-linked homodimer of the carboxy-terminal 112 amino acid residues. Mature human GDF-15 shares 66.1% and 68.7% amino acid sequence similarity with rat and mouse GDF-15, respectively, which are remarkably low homologies between species in TGF-beta superfamily. GDF-15 has been shown to have various functions, including inhibition of production of tumor necrosis factor alpha (TNF-alpha ) from lipopolysaccharide-stimulated macrophages, induction of cartilage formation, early-stage endochonadal bone formation, and promotion of neuronal survival.

1. Bootcov, M.R. et al. (1997) Proc. Natl. Acad. Sci. USA 94:11514.
2. Böttner, M. et al. (1999) Gene 237:105.
3. Fairlie, W.D. et al. (1998) J. Leukoc. Biol 65:2.
4. Fairlie, W.D. et al. (2001) J B.C 20:16911.
5. Bauskin, A.R. et al. (2000) EMBO J. 19:2212.
6. Strelau, J. et al. (2000) J. Neurosci. 20:8597.
7. Schober, A. et al. (2001) J. Comp. Neurol. 439:32.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q99988()