| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7605-SP | |||
HAF007). A specific band was detected for GCN2 at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GCN2 Monoclonal Antibody (Catalog # MAB6878) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GCN2 at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6878-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21513-50ul | |||
![Western Blot: GCN2 Antibody (6E2X0) [NBP3-16112] -Analysis of various lysates, using GCN2 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16112_rabbit-gcn2-mab-6e2x0-western-blot-206202310298..jpg "Western Blot: GCN2 Antibody (6E2X0) [NBP3-16112] -Analysis of various lysates, using GCN2 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: GCN2 Antibody (6E2X0) [NBP3-16112] - Analysis of Rat brain, using GCN2 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-16112_rabbit-gcn2-mab-6e2x0-western-blot-206202310312..jpg)
![IHC-P-GCN2 Antibody (6E2X0) [NBP3-16112] -Analysis of paraffin-embedded mouse kidney using GCN2 Rabbit mAb at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16112_rabbit-gcn2-mab-6e2x0-immunohistochemistry-paraffin-2062023103237..jpg)
![IHC-P-GCN2 Antibody (6E2X0) [NBP3-16112] - Analysis of paraffin-embedded rat intestine using GCN2 Rabbit mAb at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16112_rabbit-gcn2-mab-6e2x0-immunohistochemistry-paraffin-2062023103357..jpg)
![Immunohistochemistry-Paraffin: GCN2 Antibody [NBP3-12273] - Rat heart. 1:100 dilution in IHC blocking buffer. DAB (brown) substrate. Hematoxylin QS (blue) counter stain. 40X magnfication.](http://images.novusbio.com/fullsize/GCN2-Antibody-Immunohistochemistry-Paraffin-NBP3-12273-img0001.jpg "Immunohistochemistry-Paraffin: GCN2 Antibody [NBP3-12273] - Rat heart. 1:100 dilution in IHC blocking buffer. DAB (brown) substrate. Hematoxylin QS (blue) counter stain. 40X magnfication.")
![Western Blot: GCN2 Antibody [NBP2-38126] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/GCN2-Antibody-Western-Blot-NBP2-38126-img0003.jpg "Western Blot: GCN2 Antibody [NBP2-38126] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG")
![Immunocytochemistry/Immunofluorescence: GCN2 Antibody [NBP2-38126] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/fullsize/GCN2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38126-img0005.jpg)
![Immunohistochemistry-Paraffin: GCN2 Antibody [NBP2-38126] - Staining of human gallbladder shows weak cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/GCN2-Antibody-Immunohistochemistry-Paraffin-NBP2-38126-img0004.jpg)
![Western Blot: GCN2 Antibody [NBP3-12274] - Kidney extract. Antibody diluiton 1:500 in DiluObuffer. Apparent MW is 240 KDa.](http://images.novusbio.com/fullsize/GCN2-Antibody-Western-Blot-NBP3-12274-img0001.jpg "Western Blot: GCN2 Antibody [NBP3-12274] - Kidney extract. Antibody diluiton 1:500 in DiluObuffer. Apparent MW is 240 KDa.")
![Western Blot: GCN2 Antibody [NB100-1593] - Detection of Human GCN2 on HeLa whole cell lysate using NB100-1593. GCN2 was also immunoprecipitated using another GCN2 Ab.](http://images.novusbio.com/fullsize/GCN2-Antibody-Western-Blot-NB100-1593-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: GCN2 Antibody [NB100-1593] - Detection of Human GCN2 on HeLa whole cell lysate using NB100-1593. GCN2 was also immunoprecipitated using another GCN2 Ab.")
![Western Blot: GCN2 [p Thr 899] Antibody (SR1336) [NBP3-21513] - Western blot analysis using NBP3-21513 in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Calyculin.](http://images.novusbio.com/fullsize/nbp3-21513_rabbit-gcn2-p-thr-899-mab-sr1336-572023152468.jpg "<b>Biological Strategies Validation. </b>Western Blot: GCN2 [p Thr 899] Antibody (SR1336) [NBP3-21513] - Western blot analysis using NBP3-21513 in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Calyculin.")
