GCN2 Antibody [Alexa Fluor® 750]

• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 750

GCN2 Antibody [Alexa Fluor® 750] Summary

• Immunogen: Phosphopeptide containing human GCN2 T899 site
• Specificity: Detects human GCN2 when phosphorylated at T899 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

GCN2 (General Control Non-derepressible 2-like protein; also eIF2a kinase 4) is a 190-200 kDa member of the GCN2 subfamily, Ser/Thr kinase family, protein kinase superfamily of proteins  It is widely expressed, being found in skeletal muscle cells, fibroblasts, hepatocytes and neurons.  GCN2 is a key regulator of metabolism under nutrient deprivation.  In particular, when essential amino acids (aa) are limiting, tRNAs exist that are unbound to amino acids.  These “uncharged” tRNAs will bind to GCN2, activating the molecule.  Through GCN2-mediated phosphorylation of eIF2a, promotes GCN4 synthesis, with subsequent activation of amino acid synthesizing enzymes, and a downregulation of lipid storage.  Human GCN2 is 1649 aa in length. It possesses one RWD domain (aa 25-137), two protein kinase domains (aa 296-539 and 590-1001) and an extended His-RS-like region (aa 1021-1492).  There are at least three utilized Ser/Thr phosphorylation sites.  Two isoform variants exist.  One shows a deletion of aa 774-801, while another contains a nine aa substitution for aa 608-1649.  Over aa 22-139, human GCN2 shares 91% aa sequence identity with mouse GCN2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.