GCLC Antibody (OTI1A3)

Images

 
Western Blot: GCLC Antibody (1A3) [NBP2-45830] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCLC.
Immunohistochemistry: GCLC Antibody (1A3) [NBP2-45830] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Western Blot: GCLC Antibody (1A3) [NBP2-45830] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: ...read more
Immunohistochemistry: GCLC Antibody (1A3) [NBP2-45830] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: GCLC Antibody (1A3) [NBP2-45830] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, IHC
Clone
OTI1A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

GCLC Antibody (OTI1A3) Summary

Immunogen
Full length human recombinant protein of human GCLC(NP_001489) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GCLC
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200 - 1000
  • Immunohistochemistry 1:150
Theoretical MW
72.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-45830 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29799837).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for GCLC Antibody (OTI1A3)

  • EC 6.3.2.2
  • gamma-ECS
  • Gamma-glutamylcysteine synthetase
  • GCS heavy chain
  • GCS
  • GLCL
  • GLCLCGCL
  • glutamate--cysteine ligase catalytic subunit
  • glutamate-cysteine ligase, catalytic subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GCLC Antibody (NBP2-45830)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC, WB.


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IHC
(1)
WB
(1)
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Filter By Species
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GCLC Antibody (NBP2-45830) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70830AF350
Alexa Fluor 405 NBP2-70830AF405
Alexa Fluor 488 NBP2-70830AF488
Alexa Fluor 532 NBP2-70830AF532
Alexa Fluor 594 NBP2-70830AF594
Alexa Fluor 647 NBP2-70830AF647
Alexa Fluor 700 NBP2-70830AF700
Alexa Fluor 750 NBP2-70830AF750
Biotin NBP2-70830B
DyLight 350 NBP2-70830UV
DyLight 405 NBP2-70830V
DyLight 488 NBP2-70830G
DyLight 550 NBP2-70830R
DyLight 594 NBP2-70830DL594
DyLight 650 NBP2-70830C
DyLight 680 NBP2-70830FR
DyLight 755 NBP2-70830IR
FITC NBP2-70830F
HRP NBP2-70830H
Janelia Fluor 549 NBP2-70830JF549
Janelia Fluor 646 NBP2-70830JF646

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Blogs on GCLC.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

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Contact Information

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Bioinformatics

Gene Symbol GCLC