GCLC Antibody (OTI1A3)

Western Blot: GCLC Antibody (1A3) [NBP2-45830] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCLC.

Immunohistochemistry: GCLC Antibody (1A3) [NBP2-45830] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Western Blot: GCLC Antibody (1A3) [NBP2-45830] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: GCLC Antibody (1A3) [NBP2-45830] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: GCLC Antibody (1A3) [NBP2-45830] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, IHC
• Clone: OTI1A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

GCLC Antibody (OTI1A3) Summary

• Immunogen: Full length human recombinant protein of human GCLC(NP_001489) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GCLC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:200 - 1000
• Theoretical MW: 72.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29799837). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GCLC Antibody (OTI1A3)

• EC 6.3.2.2
• Gamma-ECS
• Gamma-glutamylcysteine synthetase
• GCLC
• GCS heavy chain
• GCS
• GLCL
• GLCLC
• GLCLCGCL
• glutamate--cysteine ligase catalytic subunit
• glutamate-cysteine ligase, catalytic subunit

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GCLC Antibody (NBP2-45830)(2)

Publications using NBP2-45830

• BessiEres B, Cruz E, Alberini CM Metabolomic profiling reveals a differential role for hippocampal glutathione reductase in infantile memory formation eLife 2021-11-26 [PMID: 34825649] (WB, Rat)
• Petersen Dennis R, Saba Laura M, Sayin Volkan I et al. Elevated Nrf-2 responses are insufficient to mitigate protein carbonylation in hepatospecific PTEN deletion mice. PloS One 2018-05-25 [PMID: 29799837] (IF/IHC, WB, Mouse)

Bioinformatics

• Gene Symbol: GCLC