GCLC Antibody (OTI1A3) [mFluor Violet 500 SE]

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, IHC
• Clone: OTI1A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 500 SE

GCLC Antibody (OTI1A3) [mFluor Violet 500 SE] Summary

• Immunogen: Full length human recombinant protein of human GCLC(NP_001489) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GCLC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for GCLC Antibody (OTI1A3) [mFluor Violet 500 SE]

• EC 6.3.2.2
• Gamma-ECS
• Gamma-glutamylcysteine synthetase
• GCLC
• GCS heavy chain
• GCS
• GLCL
• GLCLC
• GLCLCGCL
• glutamate--cysteine ligase catalytic subunit
• glutamate-cysteine ligase, catalytic subunit

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GCLC