GCKR Overexpression Lysate (Native)

Images

 
Western Blot: GCKR Overexpression Lysate (Adult Normal) [NBL1-11010] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GCKR.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GCKR Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC214230 and based on accession number NM_001486. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens glucokinase (hexokinase 4) regulator (GCKR), mRNA.
Gene
GCKR

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
68.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GCKR Overexpression Lysate (Native)

  • FGQTL5
  • GKRP
  • glucokinase (hexokinase 4) regulator
  • glucokinase (hexokinase 4) regulatory protein
  • Glucokinase regulator
  • glucokinase regulatory protein

Background

This gene encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY). [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-47778
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP1-88584
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-57033
Species: Hu
Applications: WB
NB400-139
Species: Hu, Ha
Applications: ICC/IF (-), WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NLS932
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-Fr, IHC-P
NBP1-89679
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-19083
Species: Hu
Applications: WB, ICC/IF
AF7197
Species: Hu, Mu
Applications: WB, IHC, ICC
NBP1-86616
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-55298
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP1-80533
Species: Mu
Applications: WB
NBP2-65289
Species: Hu
NBP1-88057
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-22218
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
AF5739
Species: Hu, Mu, Rt
Applications: WB

Publications for GCKR Lysate (NBL1-11010) (0)

There are no publications for GCKR Lysate (NBL1-11010).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GCKR Lysate (NBL1-11010) (0)

There are no reviews for GCKR Lysate (NBL1-11010). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GCKR Lysate (NBL1-11010) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for GCKR Lysate (NBL1-11010)

Discover related pathways, diseases and genes to GCKR Lysate (NBL1-11010). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GCKR Lysate (NBL1-11010)

Discover more about diseases related to GCKR Lysate (NBL1-11010).
 

Pathways for GCKR Lysate (NBL1-11010)

View related products by pathway.

PTMs for GCKR Lysate (NBL1-11010)

Learn more about PTMs related to GCKR Lysate (NBL1-11010).

Blogs on GCKR

There are no specific blogs for GCKR, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Glut2 Antibody
NBP2-22218

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GCKR Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol GCKR