GCDH Antibody

Western Blot: GCDH Antibody [H00002639-B01P] - Analysis of GCDH expression in human liver.

Immunocytochemistry/Immunofluorescence: GCDH Antibody [H00002639-B01P] - Analysis of purified antibody to GCDH on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: GCDH Antibody [H00002639-B01P] - Analysis of GCDH expression in transfected 293T cell line by GCDH polyclonal antibody. Lane 1: GCDH transfected lysate(48.18 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

GCDH Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: GCDH (NP_000150.1, 1 a.a. - 438 a.a.) full-length human protein. MALRGVSVRLLSRGPGLHVLRTWVSSAAQTEKGGRTQSQLAKSSRPEFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGVLGPTIKGYGCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMHPIYAYGSEEQRQKYLPQLAKGELLGCFGLTEPNSGSDPSSMETRAHYNSSNKSYTLNGTKTWITNSPMADLFVVWARCEDGCIRGFLLEKGMRGLSAPRIQGKFSLRASATGMIIMDGVEVPEENVLPGASSLGGPFGCLNNARYGIAWGVLGASEFCLHTARQYALDRMQFGVPLARNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEGTHDIHALILGRAITGIQAFTASK
• Specificity: GCDH - glutaryl-Coenzyme A dehydrogenase,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: GCDH
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It is also useful for IF.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GCDH Antibody

• ACAD5
• EC 1.3.99
• EC 1.3.99.7
• GCD
• glutaryl-CoA dehydrogenase
• glutaryl-CoA dehydrogenase, mitochondrial
• glutaryl-Coenzyme A dehydrogenase

Background

The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GCDH
• Entrez:
  • 2639(Human)
• Uniprot:
  • NP_000150.1(Human)