Human GATA3-RE Luciferase - (LUCPorter™) Stable Reporter Cell Line

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Human GATA3-RE Luciferase - (LUCPorter™) Stable Reporter Cell Line Summary

• Description: The GATA3-RE/LUCPorter™ reporter cell line can be used for screening of agonists, antagonists or signaling inhibitors related with the GATA3-involved signaling pathways. The cell line is designed to measure the transcriptional activity of GATA3. As a zinc-finger transcription factor, GATA3 (GATA-binding protein 3) plays a critical role in early and late T cell differentiation, which regulates Th1/Th2 differentiation. GATA3 has been shown to induce Th2 differentiation and repress Th1 differentiation. GATA3 is also known to promote the secretion of IL-4, IL-5 and IL-13 from Th2 cells.
  
  According to the Cancer Genome Atlas Network's study, GATA3 is one of three genes (GATA3, TP53 and PIK3CA) of which somatic mutations occurred at over 10% incidence across all breast cancers. The GATA3 induction by phorbol 12-myristate 13-acetate (PMA) is shown in Figure 1. In the test, PMA induces the transcriptional activity of GATA3 in a dose response manner. GATA3 then binds to GATA3-RE leading to expression of the luciferase reporter protein, which is quantified by luciferase activity (figure 1).
  
  Contents: 3~4 x 10^6 cells
  Biosafety Level: 2
• Immunogen: The GATA3-RE stable reporter cell line is a stably transfected HEK 293 cell line which expresses an optimized Renilla luciferase reporter gene (RenSP) under the transcriptional control of the GATA3 response element (GATA3-RE).
• Target Species: Human
• Specificity: GATA3-RE/LUCPorter™ (HEK 293)
• Selection Agent: Puromycin
• Growth Properties: Adherent Morphology: Epithelial
• RCL Type: Luciferase - (LUCPorter™)
• Reporter Gene: Renilla luciferase
• Host: HEK293
• Gene: GATA3

Applications/Dilutions

• Dilutions:
  • Ligand Activation
• Readout System:
  • LightSwitch Luciferase Assay Reagent NBP2-32066

Packaging, Storage & Formulations

• Storage: Store in gas phase of liquid nitrogen.
• Reconstitution Instructions: Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 3 ug/ml puromycin.

Details for Array

• Type: Cell

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
- Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
- No eating, drinking or smoking while handling the GATA3 reporter cell line.
- Wash hands after handling the GATA3 reporter cell line and before leaving the lab.
- Decontaminate work surface with disinfectant or 70% ethanol before and after working with the cells.
- All waste should be considered hazardous.
- Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for Human GATA3-RE Luciferase - (LUCPorter™) Stable Reporter Cell Line

• GATA3 RE
• GATA3 response element

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GATA3