GATA-5 Antibody

Images

 
Western Blot: GATA-5 Antibody [NB100-55399] - (0.3ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

GATA-5 Antibody Summary

Immunogen
Peptide with sequence C-ELAARPGWAQTATAD corresponding to internal region according to NP_536721.1.
Epitope
ELAARPGWAQTATAD
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GATA5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 40 kDa band observed in lysates of liver cell line HepG2 (calculated MW of 41.3 kDa band according to NP_536721.1).
Theoretical MW
41.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NB100-55399.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GATA-5 Antibody

  • bB379O24.1
  • GATA binding factor-5
  • GATA binding protein 5
  • GATA5
  • GATA-5
  • GATA-binding factor 5
  • GATA-binding protein 5
  • transcription factor GATA-5

Background

The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GATA-5 Antibody (NB100-55399)(1)

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GATA5
Entrez
Uniprot