Mouse GATA-4 ELISA Kit (Colorimetric)

ELISA: Mouse GATA-4 ELISA Kit (Colorimetric) [NBP3-00434] - Samples were spiked with high concentrations of Mouse GATA-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse GATA-4 ELISA Kit (Colorimetric) [NBP3-00434] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Mouse GATA-4 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse GATA-4 in samples. No significant cross-reactivity or interference between Mouse GATA-4 and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 5.45%
• Intra-Assay: 5.24%
• Spike Recovery: 87 - 105%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GATA4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse GATA-4 ELISA Kit (Colorimetric)

• GATA binding protein 4
• GATA4
• GATA-4
• GATA-binding factor 4
• GATA-binding protein 4
• MGC126629
• transcription factor GATA-4

Background

GATA4 is a 46 kDa member of the GATA family of zinc finger containing transcription factors that is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: GATA4