GATA-4 Antibody [Unconjugated]

Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GATA-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GATA-4 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

KATO‑III human gastric carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GATA‑4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human GATA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The mucin4 promoter was detected by standard PCR.

GATA‑4 was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human GATA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

GATA‑4 was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human GATA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2606) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Product Discontinued

GATA-4 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human GATA-4
  Met27-Phe211
  Accession # P43694
• Specificity: Detects human GATA-4 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GATA4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-4 Antibody [Unconjugated]

• GATA binding protein 4
• GATA4
• GATA-4
• GATA-binding factor 4
• GATA-binding protein 4
• MGC126629
• transcription factor GATA-4

Background

GATA-4 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-4 interacts with other transcription factors such as NKX2.5, and myocyte enhancer factor 2 to regulate cardiac myocyte-specific gene expression. Human GATA-4 share 93%, 93% and 92% amino acid sequence identity with porcine, rat and mouse GATA-4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GATA-4 Antibody (AF2606)(24)

Publications using AF2606

• Devito, LG;Zanjani, ZS;Evans, JR;Scardamaglia, A;Houlden, H;Gandhi, S;Healy, L; Generation of TWO G51D SNCA missense mutation iPSC lines (CRICKi011-A, CRICKi012-A) from two individuals at risk of Parkinson's disease Stem cell research 2023-06-06 [PMID: 37336145] (ICC, Human)
• K Krawczyk, K Wilczak, K Szczepa?sk, M Maleszewsk, A Suwi?ska Paracrine interactions through FGFR1 and FGFR2 receptors regulate the development of preimplantation mouse chimaeric embryo Oncogene, 2022-11-16;12(11):220193. 2022-11-16 [PMID: 36382369] (IHC, Mouse)
• LG Devito, F Cooper, I D'Angelo, J Smith, L Healy Generation of FOUR iPSC lines (CRICKi004-A; CRICKi005-A; CRICKi006-A, CRICKi007-A) from Spinal muscle atrophy patients with lower extremity dominant (SMALED) phenotype Stem Cell Research, 2022-10-28;65(0):102954. 2022-10-28 [PMID: 36332468] (ICC, Human)
• K Krawczyk, E Kosyl, K Cz??cik-?y, T Wyszomirsk, M Maleszewsk Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos Scientific Reports, 2021-11-02;11(1):21422. 2021-11-02 [PMID: 34728646] (IHC, Mouse)
• LG Devito, L Healy, S Mohammed, F Guillemot, C Dias Generation of an iPSC line (CRICKi001-A) from an individual with a germline SMARCA4 missense mutation and autism spectrum disorder Stem Cell Research, 2021-03-20;53(0):102304. 2021-03-20 [PMID: 33799280] (Flow Cytometry, ICC, Human)
• R Stavniichu, A DeLaForest, CA Thompson, J Miller, RF Souza, MA Battle GATA4 blocks squamous epithelial cell gene expression in human esophageal squamous cells Scientific Reports, 2021-02-05;11(1):3206. 2021-02-05 [PMID: 33547361] (ICC, IHC, Human)
• P Khurana, N Kolundzic, M Rogar, C Hobbs, XFCC Wong, JEA Common, D Ilic, M Liovic Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats Stem Cell Res, 2020-04-24;45(0):101827. 2020-04-24 [PMID: 32361315] (Flow Cytometry, Human)
• FM Cernilogar, S Hasenöder, Z Wang, K Scheibner, I Burtscher, M Sterr, P Smialowski, S Groh, IM Evenroed, GD Gilfillan, H Lickert, G Schotta Pre-marked chromatin and transcription factor co-binding shape the pioneering activity of Foxa2 Nucleic Acids Res., 2019-09-26;0(0):. 2019-09-26 [PMID: 31350899] (ChIP, Mouse)
• K Lee, H Cho, RW Rickert, QV Li, J Pulecio, CS Leslie, D Huangfu FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation Cell Rep, 2019-07-09;28(2):382-393.e7. 2019-07-09 [PMID: 31291575] (Immunoprecipitation, Human)
• N Kolundzic, P Khurana, L Devito, M Donne, C Hobbs, J Jeriha, XFCC Wong, JAE Common, EB Lane, S Dubrac, R Gruber, M Schmuth, TM Mauro, D Ilic Induced pluripotent stem cell line heterozygous for p.R2447X mutation in filaggrin: KCLi002-A Stem Cell Res, 2019-05-13;38(0):101462. 2019-05-13 [PMID: 31103941] (IHC, Human)
• N Kolundzic, P Khurana, C Hobbs, M Rogar, S Ropret, H Törmä, D Ilic, M Liovic Induced pluripotent stem cell (iPSC) line from an epidermolysis bullosa simplex patient heterozygous for keratin 5 E475G mutation and with the Dowling Meara phenotype Stem Cell Res, 2019-03-27;37(0):101424. 2019-03-27 [PMID: 30933721] (IHC, Human)
• L Devito, M Donne, N Kolundzic, P Khurana, C Hobbs, G Kaddour, S Dubrac, R Gruber, M Schmuth, T Mauro, D Ilic Induced pluripotent stem cell line from an atopic dermatitis patient heterozygous for c.2282del4 mutation in filaggrin: KCLi001-A Stem Cell Res, 2018-07-25;31(0):122-126. 2018-07-25 [PMID: 30075366] (ICC, Human)
• JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency Cell Rep, 2017-04-04;19(1):20-35. 2017-04-04 [PMID: 28380358] (ICC, Human)
• Inhibiting Extracellular Vesicle Release from Human Cardiosphere Derived Cells with Lentiviral Knockdown of nSMase2 Differentially Effects Proliferation and Apoptosis in Cardiomyocytes, Fibroblasts and Endothelial Cells In Vitro PLoS ONE, 2016-11-02;11(11):e0165926. 2016-11-02 [PMID: 27806113] (IHC-Fr, Mouse)
• Nagaoka M, Kobayashi M, Kawai C, Mallanna S, Duncan S Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells. PLoS ONE, 2015-08-26;10(8):e0136350. 2015-08-26 [PMID: 26308339] (IHC, Human)
• Yoon , Se-Jin, Foley , Joseph W, Baker , Julie C HEB associates with PRC2 and SMAD2/3 to regulate developmental fates. Nat Commun, 2015-03-16;6(0):6546. 2015-03-16 [PMID: 25775035] (Western Blot, Mouse)
• Tabata T, Petitt M, Zydek M, Fang-Hoover J, Larocque N, Tsuge M, Gormley M, Kauvar L, Pereira L Human cytomegalovirus infection interferes with the maintenance and differentiation of trophoblast progenitor cells of the human placenta. J Virol, 2015-03-04;89(9):5134-47. 2015-03-04 [PMID: 25741001] (IHC-P, Human)
• Shu , Jian, Zhang , Ke, Zhang , Minjie, Yao , Anzhi, Shao , Sida, Du , Fengxia, Yang , Caiyun, Chen , Wenhan, Wu , Chen, Yang , Weifeng, Sun , Yingli, Deng , Hongkui GATA family members as inducers for cellular reprogramming to pluripotency. Cell Res, 2015-01-16;25(2):169-80. 2015-01-16 [PMID: 25591928] (Immunoprecipitation, Mouse)
• Cvoro A, Devito L, Milton F, Noli L, Zhang A, Filippi C, Sakai K, Suh J, H Sieglaff D, Dhawan A, Sakai T, Ilic D, Webb P A thyroid hormone receptor/KLF9 axis in human hepatocytes and pluripotent stem cells. Stem Cells, 2015-02-01;33(2):416-28. 2015-02-01 [PMID: 25330987] (Neutralization, Human)
• Villa-Diaz L, Kim J, Lahann J, Krebsbach P Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates. Stem Cells Transl Med, 2014-10-13;3(12):1410-7. 2014-10-13 [PMID: 25313201] (ICC, Human)
• van Berlo JH, Elrod JW, Aronow BJ Serine 105 phosphorylation of transcription factor GATA4 is necessary for stress-induced cardiac hypertrophy in vivo. Proc. Natl. Acad. Sci. U.S.A., 2011-07-11;108(30):12331-6. 2011-07-11 [PMID: 21746915] (Western Blot, Mouse)
• Varelas X, Sakuma R, Samavarchi-Tehrani P, Peerani R, Rao BM, Dembowy J, Yaffe MB, Zandstra PW, Wrana JL TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal. Nat. Cell Biol., 2008-06-22;10(7):837-48. 2008-06-22 [PMID: 18568018] (ICC, Human)
• Jonckheere N, Vincent A, Perrais M, Ducourouble MP, Male AK, Aubert JP, Pigny P, Carraway KL, Freund JN, Renes IB, Van Seuningen I The human mucin MUC4 is transcriptionally regulated by caudal-related homeobox, hepatocyte nuclear factors, forkhead box A, and GATA endodermal transcription factors in epithelial cancer cells. J. Biol. Chem., 2007-06-06;282(31):22638-50. 2007-06-06 [PMID: 17553805] (ChIP, Human)
• De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking. Biotechnol. Bioeng., 2007-08-15;97(6):1617-25. 2007-08-15 [PMID: 17304565] (Western Blot, Human)

Review for GATA-4 Antibody (AF2606) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IF Human 01/07/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23658023
• Species: Human

Bioinformatics

• Gene Symbol: GATA4
• Uniprot:
  • P43694()