Western Blot: GATA-4 Antibody (JA11-41) [NBP2-66863] - Analysis of GATA4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary ...read more
Immunocytochemistry/ Immunofluorescence: GATA-4 Antibody (JA11-41) [NBP2-66863] - Staining GATA4 in PC-3M cells (Green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Flow Cytometry: GATA-4 Antibody (JA11-41) [NBP2-66863] - Analysis of Hela cells with GATA4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
TBS (pH7.4), 0.05% BSA, 40% Glycerol
0.05% Sodium Azide
Protein A purified
Alternate Names for GATA-4 Antibody (JA11-41)
GATA binding protein 4
GATA-binding factor 4
GATA-binding protein 4
transcription factor GATA-4
GATA4 is a 46 kDa member of the GATA family of zinc finger containing transcription factors that is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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