GATA-2 Antibody (2D11) - Azide and BSA Free

Western Blot: GATA-2 Antibody (2D11) [H00002624-M01] - Analysis of GATA2 expression in transfected 293T cell line by GATA2 monoclonal antibody (M01), clone 2D11.Lane 1: GATA2 transfected lysate(50.5 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: GATA-2 Antibody (2D11) [H00002624-M01] - Analysis of monoclonal antibody to GATA2 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: GATA-2 Antibody (2D11) [H00002624-M01] - Analysis of GATA2 over-expressed 293 cell line, cotransfected with GATA2 Validated Chimera RNAi ( Cat # H00002624-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GATA2 monoclonal antibody (M01), clone 2D11 (Cat # H00002624-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: GATA-2 Antibody (2D11) [H00002624-M01] - GATA2 monoclonal antibody (M01), clone 2D11 Analysis of GATA2 expression in Hela S3 NE.

ELISA: GATA-2 Antibody (2D11) [H00002624-M01] - Detection limit for recombinant GST tagged GATA2 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, KD
• Clone: 2D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GATA-2 Antibody (2D11) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GATA2 (AAH18988, 1 a.a. ~ 102 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEVAPEQPRWMAHPAVLNAQHPDSHHPGLAHNYMEPAQLLPPDEVDVFFNHLDSQGNPYYANPAHARARVSYSPAHARLTGGQMCRPHLLHSPGLPWLDGGK
• Localization: Nuclear
• Specificity: GATA2 - GATA binding protein 2
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GATA2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GATA-2 Antibody (2D11) - Azide and BSA Free

• endothelial transcription factor GATA-2
• FLJ45948
• GATA binding protein 2
• GATA2
• GATA-2
• GATA-binding protein 2NFE1B
• MGC2306

Background

The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells (Tsai et al., 1994 [PubMed 8078582]). GATA1 (MIM 305371) is essential for normal primitive and definitive erythropoiesis and is expressed at high levels in erythroid cells, mast cells, and megakaryocytes. GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells. In chicken erythroid progenitors, forced expression of GATA2 promotes proliferation at the expense of differentiation (Briegel et al., 1993 [PubMed 8504932]). GATA3 (MIM 131320) expression is restricted to T-lymphoid cells and some nonhematopoietic cell types, including embryonic stem cells.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GATA-2 Antibody (H00002624-M01)(1)

Publication using H00002624-M01

• Litzenburger UM, Buenrostro JD, Wu B et al. Single-cell epigenomic variability reveals functional cancer heterogeneity. Genome Biol 2017-01-24 [PMID: 28118844]

Bioinformatics

• Gene Symbol: GATA2
• Entrez:
  • 2624(Human)
• OMIM:
  • 137295(Human)
• Uniprot:
  • AAH18988(Human)