GATA-1 Antibody

• Reactivity: Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GATA-1 Antibody Summary

• Immunogen: A synthetic peptide corresponding to mouse GATA-1 peptide.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GATA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: This antibody has been tested by ELISA and Western Blot. Expect a band approximately 43 kDa in size corresponding to mouse GATA-1 by western blotting in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Reactivity Notes

BLAST analysis was used to suggest reactivity with this protein from mouse and rat based on 100% homology for the immunogen sequence and 93% to human. Cross reactivity with Gata1 homologues from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for GATA-1 Antibody

• Eryf1
• ERYF1GATA-binding protein 1 (globin transcription factor 1)
• GATA binding protein 1 (globin transcription factor 1)
• GATA1
• GATA-1
• GATA-1erythroid transcription factor
• GATA-binding factor 1
• GF-1
• GF1globin transcription factor 1
• NF-E1 DNA-binding protein
• NFE1erythroid transcription factor 1
• transcription factor GATA1
• XLTT

Background

GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GATA1