Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, SW480 human colorectal adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and human intestine tissue. PVDF membrane was ...read more
Gas6 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using 10 µg/mL Goat Anti-Human Gas6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF885) overnight at 4 °C. ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Gas6 Antibody [Unconjugated]
AXLLGAXL stimulatory factor
AXSFAXL receptor tyrosine kinase ligand
growth arrest-specific 6
growth arrest-specific protein 6
Gas6 (Growth Arrest Specific 6) is a multimodular protein that is upregulated by a wide variety of cell types in response to growth arrest (1). Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG) (2). Human Gas6 is a 75 kDa protein that shares 77-79% amino acid (aa) sequence identity with mouse and rat Gas6 and 43% aa sequence identity with human protein S (over the region expressed). Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the EGF-like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer (3-5). Human Gas6 interacts with both mouse and rat orthologs of these receptors (1). The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl (6). Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors (7, 8). Gas6 induces a variety of responses, including prevention of apoptosis (9), cell proliferation (10), platelet-mediated thrombosis (11), retinal epithelial cell phagocytosis of outer rod segments (12), inhibition of VEGF‑induced endothelial cell chemotaxis (13), and the differentiation and expansion of NK cell precursors (14). The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells (15). Several of these effects have been shown to require gamma -carboxylation of the Gla domain (12, 16).
Hafizi, S. and B. Dahlback (2006) FEBS J. 273:5231.
Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
Stitt, T.N. et al. (1995) Cell 80:661.
Ohashi, K. et al. (1995) J. Biol. Chem. 270:22681.
Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
Goruppi, S. et al. (1997) FEBS Lett. 415:59.
Sather, S. et al. (2007) Blood 109:1026.
Budagian, V. et al. (2005) Mol. Cell. Biol. 25:9324.
Shankar, S.L. et al. (2006) J. Neurosci. 26:5638.
Yanagita, M. et al. (2002) J. Clin. Invest. 110:239.
Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
Hall, M.O. et al. (2002) Exp. Eye Res. 75:391.
Gallicchio, M. et al. (2005) Blood 105:1970.
Caraux, A. et al. (2006) Nat. Immunol. 7:747.
Wu, Y. et al. (2005) J. Cell Sci. 118:539.
Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our Gas6 Antibody [Unconjugated] and receive a gift card or discount.