Gas6 Antibody [Unconjugated]

Images

 
There are currently no images for Gas6 Antibody (AF885).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Gas6 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Gas6
Asp118-Ala678
Accession # NP_000811
Specificity
Detects human Gas6 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse Gas6 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GAS6
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • ELISA
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF885 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Gas6 Antibody [Unconjugated]

  • AXLLG
  • AXLLGAXL stimulatory factor
  • AXSFAXL receptor tyrosine kinase ligand
  • DKFZp666G247
  • FLJ34709
  • Gas6
  • GAS-6
  • growth arrest-specific 6
  • growth arrest-specific protein 6

Background

Gas6 (Growth Arrest Specific 6) is a multimodular protein that is upregulated by a wide variety of cell types in response to growth arrest (1). Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG) (2). Human Gas6 is a 75 kDa protein that shares 77-79% amino acid (aa) sequence identity with mouse and rat Gas6 and 43% aa sequence identity with human protein S (over the region expressed). Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the EGF-like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer (3-5). Human Gas6 interacts with both mouse and rat orthologs of these receptors (1). The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl (6). Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors (7, 8). Gas6 induces a variety of responses, including prevention of apoptosis (9), cell proliferation (10), platelet-mediated thrombosis (11), retinal epithelial cell phagocytosis of outer rod segments (12), inhibition of VEGF‑induced endothelial cell chemotaxis (13), and the differentiation and expansion of NK cell precursors (14). The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells (15). Several of these effects have been shown to require gamma -carboxylation of the Gla domain (12, 16).

  1. Hafizi, S. and B. Dahlback (2006) FEBS J. 273:5231.
  2. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
  3. Stitt, T.N. et al. (1995) Cell 80:661.
  4. Ohashi, K. et al. (1995) J. Biol. Chem. 270:22681.
  5. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
  6. Goruppi, S. et al. (1997) FEBS Lett. 415:59.
  7. Sather, S. et al. (2007) Blood 109:1026.
  8. Budagian, V. et al. (2005) Mol. Cell. Biol. 25:9324.
  9. Shankar, S.L. et al. (2006) J. Neurosci. 26:5638.
  10. Yanagita, M. et al. (2002) J. Clin. Invest. 110:239.
  11. Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
  12. Hall, M.O. et al. (2002) Exp. Eye Res. 75:391.
  13. Gallicchio, M. et al. (2005) Blood 105:1970.
  14. Caraux, A. et al. (2006) Nat. Immunol. 7:747.
  15. Wu, Y. et al. (2005) J. Cell Sci. 118:539.
  16. Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF2228
Species: Hu
Applications: WB, IHC, ICC
AF591
Species: Mu
Applications: Flow, IHC, CyTOF-ready, Dual ISH-IHC
NBP1-28635
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, Flow-IC
AF5534
Species: Hu
Applications: WB, Flow, IHC
NB100-56749
Species: Hu, Mu, Rt, Rb
Applications: WB, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-47842
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, IHC, IHC-P, IP
NBP1-33116
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-15071
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KO
NBP2-19804
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-46404
Species: Hu, Mu, Rt
Applications: IHC, IHC-P
AF2656
Species: Hu
Applications: WB, IHC
H00054778-M05
Species: Hu
Applications: WB, ELISA, ICC/IF, RNAi
NBP2-50599
Species: Hu
Applications: WB, ELISA, Flow, IHC, IP, CyTOF-ready
NB600-1335
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ELISA, Flow, GS, ICC/IF, IHC, IHC-P, IP, ChIP, KD
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP1-47863
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Gas6 Antibody (AF885)(14)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 8 applications: Binding Assay, ELISA (detection), ELISA Development, ICC, IHC, IHC-Fr, Neutralization, Western Blot.


Filter By Application
Binding Assay
(1)
ELISA (detection)
(1)
ELISA Development
(3)
ICC
(2)
IHC
(1)
IHC-Fr
(1)
Neutralization
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(13)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF885 Applications Species
M Bellan, A Dimagli, C Piccinino, A Giubertoni, A Ianniello, F Grimoldi, M Sguazzotti, A Nerviani, M Barini, A Carriero, C Smirne, ME Burlone, C Rigamonti, R Minisini, L Salmi, MN Barbaglia, LM Castello, D Sola, P Marino, GC Avanzi, M Pirisi, PP Sainaghi Role of Gas6 and TAM Receptors in the Identification of Cardiopulmonary Involvement in Systemic Sclerosis and Scleroderma Spectrum Disorders Dis. Markers, 2020;2020(0):2696173. 2020 [PMID: 32454903] (ELISA (detection), Human) ELISA (detection) Human
K Fujita, X Chen, H Homma, K Tagawa, M Amano, A Saito, S Imoto, H Akatsu, Y Hashizume, K Kaibuchi, S Miyano, H Okazawa Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology Nat Commun, 2018;9(1):433. 2018 [PMID: 29382817] (Binding Assay) Binding Assay
R Kanzaki, H Naito, K Kise, K Takara, D Eino, M Minami, Y Shintani, S Funaki, T Kawamura, T Kimura, M Okumura, N Takakura Gas6 derived from cancer-associated fibroblasts promotes migration of Axl-expressing lung cancer cells during chemotherapy Sci Rep, 2017;7(1):10613. 2017 [PMID: 28878389] (Western Blot, Human) Western Blot Human
A Decker, Y Jung, FC Cackowski, K Yumoto, J Wang, RS Taichman Sympathetic signaling reactivates quiescent�disseminated prostate cancer cells in the bone marrow Mol. Cancer Res., 2017;0(0):. 2017 [PMID: 28814453] (ICC, Human) ICC Human
X Wu, W Ma, Q Zhou, H Yan, ZF Lim, M Huang, C Deng, X Yu, H Su, S Komo, H Yang, X Zhang, S Wen, Z Zhang, PC Ma AXL-GAS6 expression can predict for adverse prognosis in non-small cell lung cancer with brain metastases J. Cancer Res. Clin. Oncol., 2017;0(0):. 2017 [PMID: 28551766] (IHC, Human) IHC Human
Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis. J. Exp. Med., 2012;209(11):2033-47. 2012 [PMID: 23071254] (Western Blot, Human) Western Blot Human
Expansion of necrotic core and shedding of Mertk receptor in human carotid plaques: a role for oxidized polyunsaturated fatty acids? Cardiovasc Res, 2013;97(1):125-33. 2013 [PMID: 22997156] (Western Blot, Human) Western Blot Human
Zizzo G, Hilliard B, Monestier M, Cohen P Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol, 2012;189(7):3508-20. 2012 [PMID: 22942426] (ELISA Development, Human) ELISA Development Human
Clauser S, Meilhac O, Bieche I, Raynal P, Bruneval P, Michel JB, Borgel D Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques. Thromb. Haemost., 2011;107(1):140-9. 2011 [PMID: 22072012] (Neutralization, Human) Neutralization Human
Hurtado B, Munoz X, Recarte-Pelz P, Garcia N, Luque A, Krupinski J, Sala N, Garcia de Frutos P Expression of the vitamin K-dependent proteins GAS6 and protein S and the TAM receptor tyrosine kinases in human atherosclerotic carotid plaques. Thromb. Haemost., 2011;105(5):873-82. 2011 [PMID: 21384080] (Western Blot, Human) Western Blot Human
Show All 14 Publications.

Reviews for Gas6 Antibody (AF885) (0)

There are no reviews for Gas6 Antibody (AF885). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Gas6 Antibody (AF885) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF885

Additional Gas6 Products

Bioinformatics Tool for Gas6 Antibody (AF885)

Discover related pathways, diseases and genes to Gas6 Antibody (AF885). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Gas6 Antibody (AF885)

Discover more about diseases related to Gas6 Antibody (AF885).
 

Pathways for Gas6 Antibody (AF885)

View related products by pathway.

PTMs for Gas6 Antibody (AF885)

Learn more about PTMs related to Gas6 Antibody (AF885).

Blogs on Gas6

There are no specific blogs for Gas6, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Gas6 Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol GAS6
Uniprot