GAPDH-2 Antibody

Western blot shows lysates of human testis tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human GAPDH-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6276) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GAPDH-2 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GAPDH-2 was detected in immersion fixed paraffin-embedded sections of human testes using Goat Anti-Human GAPDH-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6276) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of sperm cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

GAPDH-2 Antibody Summary

• Immunogen: E. coli-derived recombinant human GAPDH-2
  Glu75-Ala222
  Accession # O14556
• Specificity: Detects human GAPDH-2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human GAPDH and recombinant mouse GAPDH is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GAPDHS
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GAPDH-2 Antibody

• EC 1.2.1
• EC 1.2.1.12
• GAPD2
• GAPD2HSD-35
• GAPDH2
• GAPDH-2
• GAPDH-2glyceraldehyde-3-phosphate dehydrogenase, testis-specific
• GAPDHS
• GAPDS
• glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
• HSD-35
• Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2
• Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

Background

GAPDH-2 (Glyceraldehyde-3-phosphate dehydrogenase 2; also GAPD2 and GAPDS) is a 40-44 kDa member of the GAPDH family of enzymes. Due to a high incidence of proline residues, it may migrate anomalously at 56 kDa in SDS-PAGE. GAPDH-2 is expressed only in testis as a homotetramer, where it is found in the cytoplasm bound to the sperm fibrous sheath. Unlike GAPDH that has multiple functions, GAPDH-2 appears to participate only in carbohydrate metabolism, generating energy via glycolysis for sperm motility and fertility. Human GAPDH-2 is 408 amino acids (aa) in length. There are two NAD binding sites (Asp106 and Asn388) with a catalytic region between aa 223-226. One splice variant shows a nine aa insertion after Ser220. Over aa 75-222, human GAPDH-2 shares 84% aa identity with mouse GAPDH-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GAPDHS
• Uniprot:
  • O14556(Human)